Induction of transcripts encoding a novel seven-transmembrane protein during the hypersensitive response to tobacco mosaic virus infection in tobacco plants
Induction of transcripts encoding a novel seven-transmembrane protein during the hypersensitive response to tobacco mosaic virus infection in tobacco plants
复制标题
烟草植物对烟草花叶病毒感染的过敏反应期间编码新型七跨膜蛋白的转录物的诱导
DOI：
10.1007/s00425-003-1108-2
复制
发表时间：
2003
期刊：
影响因子：
4.3
通讯作者：
H. Sano
中科院分区：
文献类型：
--
作者：
Y. Yamaguchi;H. Yoda;Kenji Akiyama;H. Sano
采用差异展示法筛选了携带N基因烟草植株对烟草花叶病毒感染的超敏反应中立即早期应答基因。温度从30°C转变到20°C后3小时，观察到特定克隆的转录本增加。该cDNA编码了一个330个氨基酸的多肽，其拓扑结构表明它是一个七跨膜蛋白，命名为Nt7TM1。直接观察烟草细胞表达nt7tm1绿色荧光蛋白融合蛋白，证实了这一点，该蛋白只迁移到质膜和内质网。RNA印迹杂交分析表明，水杨酸、茉莉酸、乙烯和过氧化氢均不能诱导Nt7TM1转录本。结果表明存在一种独特的病原体反应系统，涉及一种新的七跨膜蛋白。
Immediate early responsive genes were screened by the differential display method during the hypersensitive response upon tobacco mosaic virus infection of tobacco (Nicotiana tabacum L.) plants carrying the N gene. Three hours after temperature shift from 30 °C to 20 °C, an increase in transcripts of a particular clone was observed. The cDNA encoded a polypeptide of 330 amino acids, whose topology indicated it to be a seven-transmembrane protein, designated as Nt7TM1. This was confirmed by direct observation of cultured tobacco cells expressing an Nt7TM1–green fluorescent protein fusion protein, which migrated exclusively to the plasma membrane and the endoplasmic reticulum. RNA blot hybridization analysis indicated that Nt7TM1 transcripts were not induced by salicylic or jasmonic acids, ethylene or hydrogen peroxide. The results suggested the presence of a unique system for pathogen response involving a novel seven-transmembrane protein.