Biodegradation of aniline by Candida tropicalis AN1 isolated from aerobic granular sludge.
Biodegradation of aniline by Candida tropicalis AN1 isolated from aerobic granular sludge.
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DOI：
10.1016/s1001-0742(10)60501-3
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发表时间：
2011-12
影响因子：
6.9
通讯作者：
Dianzhan Wang;Guanyu Zheng;Shimei Wang;Dewei Zhang;Lixiang Zhou
中科院分区：
文献类型：
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作者：
Dianzhan Wang;Guanyu Zheng;Shimei Wang;Dewei Zhang;Lixiang Zhou
采用从某化工废水处理厂收集的初始活性污泥培养驯化苯胺降解菌。在驯化过程中，成功形成了能有效降解苯胺的好氧颗粒污泥，并从中分离到一株优势菌，命名为AN 1。考察了pH、温度、第二碳/氮源等因素对苯胺生物降解的影响。结果表明，菌株AN 1降解苯胺的最适条件为pH 7.0和28-35°C。在最佳pH和温度条件下，当苯胺初始浓度为400 mg/L时，降解速率可达17.8 mg/（L.hr）。进一步研究表明，添加1g/L葡萄糖或氯化铵作为第二碳源或第二氮源，可使微生物降解率从93.0%提高到95.1%~ 98.5%。然而，即使添加更多的葡萄糖或铵不能进一步促进降解过程，但延迟了菌株AN 1对苯胺的降解。通过形态特征、生理生化特征和26 S rDNA序列分析，将菌株AN 1鉴定为热带假丝酵母（Candida tropicalis）。
Aniline-degrading microbes were cultivated and acclimated with the initial activated sludge collected from a chemical wastewater treatment plant. During the acclimation processes, aerobic granular sludge being able to effectively degrade aniline was successfully formed, from which a preponderant bacterial strain was isolated and named as AN1. Effects of factors including pH, temperature, and second carbon/nitrogen source on the biodegradation of aniline were investigated. Results showed that the optimal conditions for the biodegradation of aniline by the strain AN1 were at pH 7.0 and 28–35°C. At the optimal pH and temperature, the biodegradation rate of aniline could reach as high as 17.8 mg/(L·hr) when the initial aniline concentration was 400 mg/L. Further studies revealed that the addition of 1 g/L glucose or ammonium chloride as a second carbon or nitrogen source could slightly enhance the biodegradation efficiency from 93.0% to 95.1%–98.5%. However, even more addition of glucose or ammonium could not further enhance the biodegradation process but delayed the biodegradation of aniline by the strain AN1. Based on morphological and physiological characteristics as well as the phylogenetic analysis of 26S rDNA sequences, the strain AN1 was identified as Candida tropicalis.