Acclimatization and subsequent gas exchange, water relations, survival and growth of microcultured apple plantlets after transplanting them in soil
Acclimatization and subsequent gas exchange, water relations, survival and growth of microcultured apple plantlets after transplanting them in soil
复制标题
微培苹果苗移栽至土壤后的适应和随后的气体交换、水分关系、存活和生长
DOI：
10.1111/j.1399-3054.1995.tb00831.x
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发表时间：
1995
影响因子：
6.4
通讯作者：
K. Shackel
中科院分区：
文献类型：
--
作者：
J. Díaz;E. Sutter;K. Shackel
以往的研究结果表明，叶片导度和净光合作用与水分状况（相对含水量）的组培苗和试管苗，驯化与降低叶片导度和蒸腾作用。本研究测定了驯化对植物离体移栽后水分状况的影响，以及水分状况与驯化后存活和生长的关系。以苹果（Malus pumila cv.在移植之前，通过暴露于4天逐渐降低的湿度来使它们适应。一旦植物被移植，驯化植物保持较高的相对含水量比nonaccommodizedplants，可能是因为一个较低的初始气孔导度和较少的蒸腾。高的植物水分状况，如高的相对含水量所示，似乎是植物移植后植物存活和生长的重要因素。较高的水分状况与较高的生长率和净同化率。还研究了试管苗与移植后4周的气体交换、水分关系和生长特性的差异。与试管苗相比，移栽苗的水分状况更好，叶片电导率和净光合速率也更高。也有增加的叶面积比（叶面积与植物生物量的比例）后，他们被移植，这可能是由于更高的净同化率在移植相比，在体外植物。
Previous findings indicated that leaf conductance and net photosynthesis were positively correlated with water status (relative water content) of tissue-cultured shoots and plantlets, and that acclimatization was associated with a reduction in leaf conductance and transpiration. In the present study, the effect of acclimatization on plant water status after plants were removed from culture and transplanted in soil, as well as the relation of water status with survival and growth after acclimatization were determined. Tissue-cultured plantlets (Malus pumila cv. Greensleaves) were acclimatized by exposure to a 4-day gradual reduction in humidity before they were transplanted. Once plants were transplanted, acclimatized plants maintained a higher relative water content than nonacclimatized plants, probably because of a lower initial stomatal conductance and less transpiration. High plant water status, as indicated by high relative water content, appeared to be an important factor for both plant survival and growth after the plants were transplanted. Higher water status was associated with higher growth rates and net assimilation rates. The differences in gas exchange, water relations and growth characteristics between plantlets in vitro and at 4 weeks after they were transplanted were also studied. Compared to in vitro plants, transplanted plants had a higher water status and higher leaf conductance and net photosynthesis. There was also an increase in the leaf area ratio (leaf area to plant biomass ratio) after they were transplanted which might have contributed to the higher net assimilation rate in transplanted compared to in vitro plants.