Root development of rat tooth germs implanted in the tooth socket and in the subcutaneous tissue
Root development of rat tooth germs implanted in the tooth socket and in the subcutaneous tissue
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DOI：
10.1111/j.1601-0825.2008.01450.x
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发表时间：
2008-10-01
期刊：
影响因子：
3.8
通讯作者：
Sasaki, K.
中科院分区：
文献类型：
--
作者：
Ina, Y.;Sasano, Y.;Sasaki, K.
目的：本研究的目的是探讨根的发展，大鼠牙胚植入在牙槽或在皮下region.Materials和方法：牙胚的左上第一磨牙在2周龄大鼠被提取和植入在原来的牙槽或在皮下区域的背部。右上第一磨牙作为对照。在第1、2、4、8和12周固定大鼠。用X射线摄影测量法对牙根发育进行定量检测。生产基质蛋白的细胞活性进行了评估，使用原位杂交I型collagen.Results：根发育观察到在植入的牙齿中的牙槽，也在对照牙齿。相比之下，根几乎没有发展在皮下植入的牙齿。组织学检查显示，牙周韧带在牙槽中的植入牙齿的牙根周围排列，与对照牙齿周围一样，但在皮下植入中很少发现牙周韧带。牙本质和牙骨质形成在两个植入的牙齿，也在控制牙齿和成牙本质细胞，成牙骨质细胞和牙骨质细胞表达I型collagen.CONCLUSION：牙槽可能具有特定的环境，允许牙根发育的牙胚。
OBJECTIVE: This study was designed to investigate root development of a rat tooth germ implanted in a tooth socket or in a subcutaneous region.MATERIALS AND METHODS: Tooth germs of the upper left first molars in 2-week-old rats were extracted and implanted in the original tooth socket or in the subcutaneous region of the back. The upper right first molar was used as a control. The rats were fixed in weeks 1, 2, 4, 8 and 12. The root development was examined quantitatively with X-ray radiographic morphometry. The cellular activity of producing matrix proteins was assessed using in situ hybridization for type I collagen.RESULTS: Root development was observed in the implanted teeth in the tooth socket as also in the control teeth. In contrast, roots hardly developed in subcutaneously implanted teeth. Histology showed that periodontal ligaments were arranged around roots of implanted teeth in the tooth socket as around control teeth, but few periodontal ligaments were identified in the subcutaneous implantation. Dentin and cementum formed in both the implanted teeth as also in the control teeth and odontoblasts, cementoblasts and cementocytes expressed type I collagen.CONCLUSION: Tooth sockets may possess specific environments that allow root development of a tooth germ.