Natural killer cells do not mediate facial motoneuron survival after facial nerve transection
Natural killer cells do not mediate facial motoneuron survival after facial nerve transection
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DOI：
10.1016/s0889-1591(03)00089-8
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发表时间：
2003-12-01
影响因子：
15.1
通讯作者：
Jones, KJ
中科院分区：
文献类型：
--
作者：
Byram, SC;Serpe, CJ;Jones, KJ
本研究的目的是确定自然杀伤（NK）细胞是否介导损伤后面运动神经元（FMN）的存活。野生型（WT）、穿孔素/重组酶激活基因-2敲除（pfp/RAG-2 KO）和普通γ链（gammac）/RAG-2 KO小鼠接受右侧面神经轴突切断术。在WT小鼠中，相对于对侧对照侧，FMN存活率为86 +/- 1.0%。相比之下，pfp/RAG-2和gammac/RAG-2 KO小鼠的FMN存活率相对于WT显著降低（分别为20%和30%）。pfplRAG-2和gammac/RAG-2 KO小鼠单独用正常NK细胞重建，不能将FMN存活水平恢复到WT的水平，但确实恢复了针对YAG-1细胞的功能性裂解活性。用脾细胞重建pfp/RAG-2和gammac/RAG-2 KO小鼠，以及用单独的CD 4（+）T淋巴细胞或与NK细胞组合重建pfp/RAG-2 KO小鼠，使FMN存活水平恢复到WT的水平。因此，NK细胞似乎不是免疫细胞介导的运动神经元免于轴突切断诱导的细胞死亡的拯救的组分。(C)2003 Elsevier Science（美国）。All rights reserved.
The goal of the current study was to determine if natural killer (NK) cells mediate facial motoneuron (FMN) survival following injury. Wild-type (WT), perforin/recombinase activating gene-2 knockout (pfp/RAG-2 KO), and common gamma-chain (gammac)/RAG-2 KO mice received a right facial nerve axotomy. In WT mice, FMN survival was 86 +/- 1.0% relative to the contralateral control side. In contrast, pfp/RAG-2 and gammac/RAG-2 KO mice exhibited significant decreases in FMN survival (similar to20% and similar to30%, respectively), relative to WT. Reconstitution of pfplRAG-2 and gammac/RAG-2 KO mice with normal NK cells alone, failed to restore FMN survival levels to those of WT, but did restore functional lytic activity against YAG-1 cells. Reconstitution of pfp/RAG-2 and gammac/RAG-2 KO mice with splenocytes, and pfp/RAG-2 KO mice with CD4(+) T-lymphocytes alone or in combination with NK cells, restored FMN survival levels to those of WT. Thus, NK cells appear to not be a component of immune cell-mediated rescue of motoneurons from axotomy induced cell death. (C) 2003 Elsevier Science (USA). All rights reserved.