Design of a new multi-epitope vaccine against Brucella based on T and B cell epitopes using bioinformatics methods.
Design of a new multi-epitope vaccine against Brucella based on T and B cell epitopes using bioinformatics methods.
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利用生物信息学方法设计基于T和B细胞表位的新型布鲁氏菌多表位疫苗
DOI：
10.1017/s0950268821001229
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发表时间：
2021-05-25
影响因子：
4.2
通讯作者：
Ding J
中科院分区：
文献类型：
--
作者：
Chen Z;Zhu Y;Sha T;Li Z;Li Y;Zhang F;Ding J
摘要布鲁氏菌病是危害最严重、分布最广的人畜共患病之一，严重威胁着人类健康和国民经济。本研究基于布鲁氏菌外膜蛋白22（Omp 22）、外膜蛋白19（Omp 19）和外膜蛋白28（Omp 28）的T/B优势表位，利用生物信息学方法设计安全有效的多表位疫苗。蛋白质的氨基酸序列在美国国家生物技术信息中心（NCBI）数据库中找到，信号肽由SignaIP-5.0服务器预测。用ProtScale软件分析蛋白质的表面可及性和亲水区域，用I-TASSER软件预测蛋白质的三级结构模型，并用UCSF Chimera软件标记。用COBEpro、SVMTriP和BepiPred软件预测蛋白的B细胞表位。用SYFpep Ⅰ、RANKpep和IEDB预测蛋白的T细胞表位。将三种蛋白质的T/B优势表位与HEYGAALEREAG和GGGS接头组合，并在EAAAK接头的帮助下将载体序列连接到疫苗构建体的N-和C-末端。最后，分析了多表位疫苗构建体的三级结构和理化性质。多表位疫苗构建体的致敏性、抗原性和溶解性分别为7.37-11.30、0.788和0.866。模拟疫苗构建体的Ramachandran图显示96.0%的残基在有利和允许的范围内。结果表明，该多表位疫苗构建体具有良好的结构和适宜的特性，为进一步的实验研究提供了理论基础。
Abstract Brucellosis is one of the most serious and widespread zoonotic diseases, which seriously threatens human health and the national economy. This study was based on the T/B dominant epitopes of Brucella outer membrane protein 22 (Omp22), outer membrane protein 19 (Omp19) and outer membrane protein 28 (Omp28), with bioinformatics methods to design a safe and effective multi-epitope vaccine. The amino acid sequences of the proteins were found in the National Center for Biotechnology Information (NCBI) database, and the signal peptides were predicted by the SignaIP-5.0 server. The surface accessibility and hydrophilic regions of proteins were analysed with the ProtScale software and the tertiary structure model of the proteins predicted by I-TASSER software and labelled with the UCSF Chimera software. The software COBEpro, SVMTriP and BepiPred were used to predict B cell epitopes of the proteins. SYFPEITHI, RANKpep and IEDB were employed to predict T cell epitopes of the proteins. The T/B dominant epitopes of three proteins were combined with HEYGAALEREAG and GGGS linkers, and carriers sequences linked to the N- and C-terminus of the vaccine construct with the help of EAAAK linkers. Finally, the tertiary structure and physical and chemical properties of the multi-epitope vaccine construct were analysed. The allergenicity, antigenicity and solubility of the multi-epitope vaccine construct were 7.37–11.30, 0.788 and 0.866, respectively. The Ramachandran diagram of the mock vaccine construct showed 96.0% residues within the favoured and allowed range. Collectively, our results showed that this multi-epitope vaccine construct has a high-quality structure and suitable characteristics, which may provide a theoretical basis for future laboratory experiments.