XCE, a new member of the endothelin-converting enzyme and neutral endopeptidase family, is preferentially expressed in the CNS
XCE, a new member of the endothelin-converting enzyme and neutral endopeptidase family, is preferentially expressed in the CNS
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DOI：
10.1016/s0169-328x(98)00321-0
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发表时间：
1999-02-05
期刊：
影响因子：
--
通讯作者：
Schweizer, A
中科院分区：
文献类型：
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作者：
Valdenaire, O;Richards, JG;Schweizer, A
在本研究中，我们已经分离出一个编码锌金属肽酶家族的新成员的cDNA，该家族包括中性内肽酶和内皮素转化酶。这种酶的预测的氨基酸序列，称为XCE，由775个氨基酸组成的一个单一的假定跨膜区，一个N-末端的59个残基的胞质结构域，和一个大的管腔域，包含一个特征性的锌结合基序。稳定表达这种新的金属肽酶的细胞的蛋白质印迹分析显示约95 kDa的糖基化蛋白。发现XCE mRNA主要在中枢神经系统、交感神经节和子宫上皮下细胞中表达。在大鼠和人类中枢神经系统中，一个非常具体的模式的神经元标记（在假定的胆碱能中间神经元的基底神经节，基底前脑神经元，以及脑干和脊髓运动神经元）进行了检测，通过原位杂交组织化学。酶底物，尚未确定，可能会发现在众多的神经肽递质，这是共定位与乙酰胆碱在这些神经元。(C)1999 Elsevier Science B. V.保留所有权利。
In the present study, we have isolated a cDNA encoding a novel member of the family of zinc metallopeptidases that includes neutral endopeptidase and endothelin-converting enzyme. The predicted amino-acid sequence of this enzyme, termed XCE, consists of 775 amino-acids with a single putative membrane-spanning region, an N-terminal cytoplasmic domain of 59 residues, and a large luminal domain that contains a characteristic zinc-binding motif. Western blot analysis of cells stably expressing this new metallopeptidase revealed a glycosylated protein of approximately 95 kDa. XCE mRNA was found to be predominantly expressed in the central nervous system, sympathetic ganglia and in uterine subepithelial cells. In the rat and human CNS, a very specific pattern of neuronal labelling (in presumptive cholinergic interneurons of basal ganglia, basal forebrain neurons, as well as brainstem and spinal cord motoneurons) was detected by in situ hybridization histochemistry. The enzyme substrate, as yet unidentified, might be found among the numerous neuropeptide transmitters which are colocalized with acetylcholine in these neurons. (C) 1999 Elsevier Science B.V. All rights reserved.