Nitrogen-doped carbon dots rapid and selective detection of mercury ion and biothiol and construction of an IMPLICATION logic gate
Nitrogen-doped carbon dots rapid and selective detection of mercury ion and biothiol and construction of an IMPLICATION logic gate
复制标题
氮掺杂碳点快速选择性检测汞离子和生物硫醇并构建蕴含逻辑门
DOI：
10.1016/j.talanta.2018.09.114
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发表时间：
2019-03-01
期刊：
影响因子：
6.1
通讯作者：
Chen, Xiaoqing
中科院分区：
文献类型：
--
作者：
Liao, Sen;Li, Xinzong;Chen, Xiaoqing
通过调节碳源（柠檬酸）和氮源（乙醇胺）的比例制备了氮掺杂碳量子点（N-CD）。该N-CDs荧光探针已成功应用于快速、灵敏、选择性地测定汞离子（Hg 2+）的浓度，线性范围为0.1-5.0 M，检测限为0.019。本工作证明，当柠檬酸的羧基与乙醇胺的氨基完全反应时，N-CDs对Hg 2+产生荧光响应。此外，在HEPES缓冲液中，半胱氨酸（Cys）、同型半胱氨酸（Hcy）和谷胱甘肽（GSH）等生物硫醇对N-CDs/Hg ~（2+）体系的猝灭有较好的回收作用，对Cys和Hcy的回收率高于GSH。N-CDs具有低毒性和良好的生物相容性，可作为一种潜在的荧光生物成像材料。在此基础上，构建了基于Hg ~（2+）和双硫醇的IMPLICATION逻辑门。
Nitrogen-doped carbon dots (N-CDs) were prepared by adjusting the ratio of carbon sources (citric acid) and nitrogen sources (ethanolamine). This N-CDs fluorescent probe had been successfully applied to rapidly, sensitively and selectively determine the concentration of mercury ion (Hg2+) with a linear range of 0.1-5.0 M and a limit of detection of 0.019 In this work, it was demonstrated that N-CDs show the fluorescence response toward Hg2+ when the carboxyl groups of citric acid reacts completely with the amino groups of ethanolamine. In addition, the quenched N-CDs/Hg2+ system could be recovered by biothiol, such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), exhibiting a sensitive response toward Cys and Hcy over GSH in HEPES buffer. N-CDs could be used as a potential fluorescent bioimaging material due to their low toxicity and good biocompatibility. Furthermore, an IMPLICATION logic gate based on Hg2+ and biothiol was constructed.