Fabrication, Mechanical Properties, and Biocompatibility of Graphene-Reinforced Chitosan Composites
Fabrication, Mechanical Properties, and Biocompatibility of Graphene-Reinforced Chitosan Composites
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DOI：
10.1021/bm100470q
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发表时间：
2010-09-01
影响因子：
6.2
通讯作者：
Jin, Zhaoxia
中科院分区：
文献类型：
--
作者：
Fan, Hailong;Wang, Lili;Jin, Zhaoxia
使用NH3作为缓冲气体之一，通过直流电弧放电法合成的多层石墨烯片分散在壳聚糖/乙酸溶液中。 FTIR和X射线光电子能谱显示石墨烯片表面存在含氧官能团，这可能有助于石墨烯在壳聚糖溶液中的良好分散。采用溶液流延法制备石墨烯/壳聚糖薄膜。采用纳米压痕法测试了复合薄膜的力学性能。在壳聚糖中添加少量石墨烯（0.1-0.3 wt%），壳聚糖的弹性模量增加了约200%。通过基于四唑的体外比色测定检查石墨烯/壳聚糖复合膜的生物相容性。细胞粘附结果表明，L929细胞能够在石墨烯/壳聚糖复合膜以及纯壳聚糖膜上粘附并发育，表明石墨烯/壳聚糖复合材料具有良好的生物相容性。由于石墨烯原料中不含金属杂质，因此在石墨烯用于制备生物医用材料时，避免了去除碳纳米管中捕获的金属纳米粒子的耗时的纯化过程。石墨烯/壳聚糖复合材料是组织工程中支架材料的潜在候选者。
Few-layered graphene sheets, synthesized by direct current arc-discharge method using NH3 as one of the buffer gases, were dispersed in chitosan/acetic acid solutions. FTIR and X-ray photoelectron spectroscopy showed the presence of oxygen-containing functional groups on the surface of graphene sheets that may assist the good dispersion of graphene in chitosan solution. Graphene/chitosan films were produced by solution casting method. The mechanical properties of composite films were tested by nanoindentation method. With the addition of a small amount of graphene in chitosan (0.1-0.3 wt %), the elastic modulus of chitosan increased over similar to 200%. The biocompatibility of graphene/chitosan composite films was checked by tetrazolium-based colorimetric assays in vitro. The cell adhesion result showed that the L929 cell can adhere to and develop on the graphene/chitosan composite films as well as on pure chitosan film, indicating that graphene/chitosan composites have good biocompatibility. Because there is no metallic impurity in graphene raw materials, the time-consuming purification process for removing metal nanoparticles entrapped in carbon nanotubes is thus avoided when graphene is used to prepare biomedical materials. Graphene/chitosan composites are potential candidates as scaffold materials in tissue engineering.