OsDOG1L-3 regulates seed dormancy through the abscisic acid pathway in rice
OsDOG1L-3 regulates seed dormancy through the abscisic acid pathway in rice
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OsDOG1L-3 通过脱落酸途径调节水稻种子休眠
DOI：
10.1016/j.plantsci.2020.110570
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发表时间：
2020
期刊：
影响因子：
5.2
通讯作者：
Wan Jianmin
中科院分区：
文献类型：
--
作者：
Wang Qian;Lin Qibing;Wu Tao;Duan Erchao;Huang Yunshuai;Yang Chunyan;Mou Changling;Lan Jie;Zhou Chunlei;Xie Kun;Liu Xi;Zhang Xin;Guo Xiuping;Wang Jie;Jiang Ling;Wan Jianmin
种子休眠与收获前发芽抗性密切相关。植物激素脱落酸 (ABA) 和延迟发芽 1 (DOG1) 蛋白都是种子休眠的关键调节因子。它们的关系在拟南芥中已被广泛报道，但在水稻中却知之甚少。在这里，我们发现数量性状基因座 qSd-1-1 对强休眠籼稻品种 N22 和非休眠粳稻品种南京 35 之间的种子休眠差异有显着影响。它编码一种名为 OsDOG1L-3 的 DOG1 样蛋白，与拟南芥 DOG1 同源。 N22 和南京 35 之间 OsDOG1L-3 的启动子和表达存在明显差异，南京 35 中 N22 OsDOG1L-3 等位基因的过表达或引入增强了其种子休眠。 OsDOG1L-3 表达与种子休眠呈正相关并受 ABA 诱导。 OsbZIP75和OsbZIP78直接与OsDOG1L-3的启动子结合以诱导其表达。 OsbZIP75 的过度表达增加了 OsDOG1L-3 蛋白丰度并促进种子休眠。 OsDOG1L-3上调ABA相关基因的表达并增加ABA含量。我们提出N22 OsDOG1L-3等位基因是QTL qSd-1-1中种子休眠的候选基因，并且它参与ABA途径以建立水稻种子休眠。
Seed dormancy is closely related to pre-harvest sprouting resistance. Both plant hormone abscisic acid (ABA) and DELAY OF GERMINATION 1 (DOG1) protein are key regulators of seed dormancy. Their relationship is well reported in Arabidopsis, but little is known in rice. Here, we show that a quantitative trait locus, qSd-1-1 con-tributes significantly to seed dormancy differences between the strongly dormant indica variety N22 and non-dormant japonica variety Nanjing35. It encodes a DOG1-like protein named OsDOG1L-3 with homology to Arabidopsis DOG1. There were evident promoter and expression differences in OsDOG1L-3 between N22 and Nanjing35, and overexpression or introduction of the N22 OsDOG1L-3 allele in Nanjing35 enhanced its seed dormancy. OsDOG1L-3 expression was positively correlated with seed dormancy and induced by ABA. OsbZIP75 and OsbZIP78 bound directly with the promoter of OsDOG1L-3 to induce its expression. Overexpression of OsbZIP75 increased OsDOG1L-3 protein abundance and promoted seed dormancy. OsDOG1L-3 upregulated expression of ABA-related genes and increased ABA content. We propose that the N22 OsDOG1L-3 allele is a candidate gene for the seed dormancy in QTL qSd-1-1, and that it participates in the ABA pathway to establish seed dormancy in rice.