Micro-CT observations of the 3D distribution of calcium oxalate crystals in cotyledons during maturation and germination in Lotus miyakojimae seeds.
Micro-CT observations of the 3D distribution of calcium oxalate crystals in cotyledons during maturation and germination in Lotus miyakojimae seeds.
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DOI：
10.1093/jmicro/dfs079
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发表时间：
2013-06
期刊：
影响因子：
1.8
通讯作者：
D. Yamauchi;Daisuke Tamaoki;Masato Hayami;M. Takeuchi;I. Karahara;Mayuko Sato;K. Toyooka;H. Nishioka;Y. Terada;K. Uesugi;H. Takano;Y. Kagoshima;Y. Mineyuki
中科院分区：
文献类型：
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作者：
D. Yamauchi;Daisuke Tamaoki;Masato Hayami;M. Takeuchi;I. Karahara;Mayuko Sato;K. Toyooka;H. Nishioka;Y. Terada;K. Uesugi;H. Takano;Y. Kagoshima;Y. Mineyuki
豆科植物种子的子叶是种子萌发和幼苗生长所需营养物质的贮藏器官。子叶内降解过程的空间和时间控制尚未阐明。草酸钙（CaOx）晶体是植物中常见的钙存款，经常被报道存在于豆类种子中。在这项研究中，微型计算机断层扫描（micro-CT）在SPring-8设施，检查三维分布的晶体子叶内的种子成熟和发芽的百脉根宫古岛（以前百脉根登录宫古岛MG-20）。利用该技术，我们可以检测胚的轮廓，种子中的空隙空间和子叶脉序模式。我们发现了几个网站，强烈抑制子叶内的X射线传输。光学和偏光显微镜证实，这些区域对应于CaOx晶体。干种子的三维观察表明，L。宫小路前子叶沿着侧脉分布，但其分布仅限于原形成层的远轴侧。CaOx晶体出现在种子发育的第二阶段（种子灌浆期），其数量在干种子中增加。子叶中的晶体的数量是高的萌发过程中，这表明CaOx晶体不降解的钙供应。CaOx晶体在L.草酸水平的生物化学测量也支持宫古岛前发芽过程。
The cotyledon of legume seeds is a storage organ that provides nutrients for seed germination and seedling growth. The spatial and temporal control of the degradation processes within cotyledons has not been elucidated. Calcium oxalate (CaOx) crystals, a common calcium deposit in plants, have often been reported to be present in legume seeds. In this study, micro-computed tomography (micro-CT) was employed at the SPring-8 facility to examine the three-dimensional distribution of crystals inside cotyledons during seed maturation and germination of Lotus miyakojimae (previously Lotus japonicus accession Miyakojima MG-20). Using this technique, we could detect the outline of the embryo, void spaces in seeds and the cotyledon venation pattern. We found several sites that strongly inhibited X-ray transmission within the cotyledons. Light and polarizing microscopy confirmed that these areas corresponded to CaOx crystals. Three-dimensional observations of dry seeds indicated that the CaOx crystals in the L. miyakojimae cotyledons were distributed along lateral veins; however, their distribution was limited to the abaxial side of the procambium. The CaOx crystals appeared at stage II (seed-filling stage) of seed development, and their number increased in dry seeds. The number of crystals in cotyledons was high during germination, suggesting that CaOx crystals are not degraded for their calcium supply. Evidence for the conservation of CaOx crystals in cotyledons during the L. miyakojimae germination process was also supported by the biochemical measurement of oxalic acid levels.