Characterization and bone differentiation of nanoporous structure fabricated on Ti6Al4V alloy
Characterization and bone differentiation of nanoporous structure fabricated on Ti6Al4V alloy
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DOI：
10.1155/2015/358951
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发表时间：
2015
影响因子：
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通讯作者：
Yingmin Su;S. Komasa;T. Sekino;H. Nishizaki;J. Okazaki
中科院分区：
文献类型：
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作者：
Yingmin Su;S. Komasa;T. Sekino;H. Nishizaki;J. Okazaki
确定了碱处理和后续热处理的最佳温度，以优化Ti6Al4V钛合金板上形成的纳米多孔结构。通过扫描电子显微镜、原子力显微镜、X射线光电子能谱和X射线衍射对碱热处理样品进行表面表征。评估加热温度对白蛋白粘附、大鼠骨髓间充质干细胞（BMMSCs）粘附、碱性磷酸酶活性、骨钙素产生、钙沉积和Runx2 mRNA表达的影响。即使在200、400和600°C下热处理后，纳米形貌、表面化学和表面粗糙度也没有变化。只有无定形的钛酸钠相改变，随着热处理的温度增加，这在促进纳米多孔表面上的上级细胞粘附中起着至关重要的作用，与通过单一碱处理获得的钛酸氢钠相比。800°C热处理并没有增强细胞在表面上的附着，因为纳米结构随着Al和V的重新出现而被显著破坏。本研究表明，通过改进的碱热处理工艺，在Ti6Al4V表面制备了具有无定形钛酸钠的纳米多孔结构，以改善BMMSCs的附着。
The optimal temperature for the alkaline treatment and subsequent heat treatment is determined to optimize the nanoporous structures formed on Ti6Al4V titanium alloy plates. Surface characterization of the alkali-heat treated samples was performed by scanning electron microscopy, atomic force microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction. The effects of heating temperatures on albumin adhesion, rat bone marrow mesenchymal stem cells (BMMSCs) adhesion, alkaline phosphatase activity, osteocalcin production, calcium deposition, and Runx2 mRNA expression were evaluated. The nanotopography, surface chemistry, and surface roughness were unchanged even after heat treatments at 200, 400, and 600°C. Only the amorphous sodium titanate phase changed, increasing with the temperature of the heat treatments, which played a crucial role in promoting superior cell adhesion on the nanoporous surface compared with the sodium hydrogen titanate obtained by a single alkali treatment. The heat treatment at 800°C did not enhance cell attachment on the surface because the nanostructure was dramatically destroyed with the reappearance of Al and V. This study reveals that nanoporous structures with amorphous sodium titanate were fabricated on Ti6Al4V surface through an amended alkali-heat treatment process to improve BMMSCs adhesion.