MOLECULAR-CLONING AND SEQUENCE DETERMINATION OF DA STRAIN OF THEILER MURINE ENCEPHALOMYELITIS VIRUSES
MOLECULAR-CLONING AND SEQUENCE DETERMINATION OF DA STRAIN OF THEILER MURINE ENCEPHALOMYELITIS VIRUSES
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DOI：
10.1016/0042-6822(88)90642-3
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发表时间：
1988-05-01
期刊：
影响因子：
3.7
通讯作者：
ROOS, RP
中科院分区：
文献类型：
--
作者：
OHARA, Y;STEIN, S;ROOS, RP
泰勒氏鼠脑脊髓炎病毒（TMEV）属于小核糖核酸病毒科，分为两个亚组。TO亚群菌株在小鼠中产生持续性脱髓鞘中枢神经系统感染，而GDVII亚群菌株引起急性脊髓灰质炎脑脊髓炎。我们获得了TO亚群成员DA株基因组的三个重叠克隆。序列分析显示，该基因组长8093个核苷酸，带有一个poly（A）尾。4“非编码区从核苷酸1延伸到1065，并且缺乏多聚（C）区。该基因的开放阅读框长度为1066 ~ 7968，编码2301个氨基酸。DA株序列与小核糖核酸病毒属的成员比与其他小核糖核酸病毒属的成员更密切相关。与另一株TO亚群BeAn株的序列比较，发现P1区的差异最大，而非编码区的保守性较好。这三个重叠克隆在两个亚组菌株之间的重组感染性cDNA研究中将是重要的。
Theiler''s murine encephalomyelitis viruses (TMEV) belong to the Picornaviridae, and are divided into two subgroups. TO subgroup strains produces a persistent demyelinating central nervous system infection in mice, while GDVII subgroup strains cause acute polioencephalomyelitis. We generated three overlapping clones of the genome of DA strain, a member of TO subgroup. Sequence analysis revealed that the genome is 8093 nucleotides long with a poly(A) tail. The 4'' noncoding region stretches from nucleotides 1 to 1065 and lacks a poly(C) tract. The open reading frame stretches from 1066 to 7968 and encodes 2301 amino acids. DA strain sequence is more closely related to members of the Cardiovirus genus than to members of other Picornavirus genera. Comparison with sequence of BeAn strain, another TO subgroup strain, showed tht the P1 area has the greatest number of differences, while the noncoding regions are more well-conserved. The three overlapping clones will be important in recombinant infectious cDNA studies between strains of both subgroups.