Effects of vitamin E on the concentration of conjugated linoleic acids and accumulation of intermediates of ruminal biohydrogenation in vitro
Effects of vitamin E on the concentration of conjugated linoleic acids and accumulation of intermediates of ruminal biohydrogenation in vitro
复制标题
维生素E对体外共轭亚油酸浓度及瘤胃生物氢化中间产物积累的影响
DOI：
10.1016/j.smallrumres.2012.09.015
复制
发表时间：
2013-04-01
影响因子：
1.8
通讯作者：
Liu, Fei
中科院分区：
文献类型：
--
作者：
Hou, Juncai;Wang, Fang;Liu, Fei
The aim of this study was to investigate effects of vitamin E (Vit. E) addition to high soybean oil diets on the concentration of conjugated linoleic acids (CLA) and accumulation of biohydrogenation intermediates in mature goat rumen fluid using an in vitro batch culture technique. Rumen fluid collected from six fistulated mature goats was incubated for 24h with addition of Vit. Eat different concentrations [0 (control), 0.5, 1.0 and 2.0 mg/80 ml] in triplicate. Results indicate that acetate, total volatile fatty acid (VFA), as well as the ratio of acetate to propionate, increased at a decreasing rate (linearly P < 0.01 and quadratic P < 0.05) with increased Vit. E supplementation, whereas the concentration of butyrate linearly (P < 0.001) decreased. Vit. E had no effect on pH, ammonia-N, and total gas production. However, Vit. E linearly (P < 0.001) decreased cis-9, trans-11 CLA concentration, linearly (P = 0.002) increased trans-11 C18:1 and concentration of trans-10 C18:1 was the lowest at intermediate addition levels of Vit. E (quadratic P < 0.001). Concentrations of linoleic acid and linolenic acid were both linearly (P < 0.05) decreased at a decreasing rate with increased Vit. E addition. Vit. E linearly (P = 0.001) decreased the oleic acid concentration, whereas that of stearic acid was linearly (P < 0.001) increased. Results demonstrate that Vit. E supplementation affected ruminal fermentation parameters, particularly the concentration of conjugated linoleic acids and accumulation of intermediates of ruminal biohydrogenation in the ruminal fluid. The mechanism by which Vit. E may alter biohydrogenation is unclear, but the modification of rumen microbial populations/dynamics and consequent fatty acid hydration might be involved. Crown Copyright (C) 2012 Published by Elsevier B.V. All rights reserved.
