CHARACTERIZATION OF THE DNA OF A DEFECTIVE HUMAN PARVOVIRUS ISOLATED FROM A GENITAL SITE
CHARACTERIZATION OF THE DNA OF A DEFECTIVE HUMAN PARVOVIRUS ISOLATED FROM A GENITAL SITE
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DOI：
10.1016/0042-6822(84)90271-x
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发表时间：
1984-01-01
期刊：
影响因子：
3.7
通讯作者：
HAUSEN, HZ
中科院分区：
文献类型：
--
作者：
BANTELSCHAAL, U;HAUSEN, HZ
从阴茎扁平湿疣病变中分离出一种明显的辅助病毒依赖性细小病毒，方法是将该材料接种到人成纤维细胞培养物中，并用12型腺病毒共感染这些细胞。在添加腺病毒辅助物后，病毒可以容易地在人口腔癌KB细胞中增殖。其DNA的分析显示，它与迄今为止描述的4种缺陷型细小病毒（腺相关病毒，AAV）的DNA具有结构相似性，但用限制性酶消化DNA后获得的切割模式与AAV 1-AAV 4的切割模式不同。核酸杂交数据进一步支持分离的缺陷型细小病毒与其他4种血清型不相同的假设。当使用高严格性条件时，仅观察到与AAV 1-AAV 4的DNA的弱交叉反应性;后者显示彼此的强交叉杂交。通过绘制几种限制性内切酶的切割位点，研究了新分离的细小病毒DNA的组织。建议将新分离株命名为AAV 5。
An apparently helper-dependent parvovirus was isolated from a penile flat condylomatous lesion by inoculating the material into a culture of human fibroblasts, and by coinfecting these cells with adenovirus type 12. Upon addition of an adenovirus helper, the virus could be readily propagated in human oral carcinoma KB cells. Analysis of its DNA revealed that it shares structural similarities with the DNA of the 4 defective parvoviruses (adeno-associated viruses, AAV) described so far, but that the cleavage pattern obtained after digesting the DNA with restriction enzymes is different from that of AAV 1-AAV 4. Nucleic acid hybridization data further support the assumption that the isolated defective parvovirus is not identical with the other 4 serotypes. When conditions of high stringency were used, only weak cross-reactivity was seen with the DNA of AAV 1-AAV 4; the latter showed strong cross-hybridization with each other. The organization of the DNA of the newly isolated parvovirus was investigated by mapping the cleavage sites of several restriction enzymes. It is proposed to designate the new isolate as AAV 5.