Comparative Evaluation of Genetically Encoded Voltage Indicators
Comparative Evaluation of Genetically Encoded Voltage Indicators
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DOI：
10.1016/j.celrep.2018.12.088
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发表时间：
2019-01-15
期刊：
影响因子：
8.8
通讯作者：
Yuste, Rafael
中科院分区：
文献类型：
--
作者：
Bando, Yuki;Sakamoto, Masayuki;Yuste, Rafael
使用荧光传感器的成像电压可能是一种理想的技术，以高时空分辨率探测神经回路。然而，由于信噪比（SNR）不足，在哺乳动物制备的成像膜电位仍然是具有挑战性的。近年来，已经开发了许多遗传编码电压指示器（GEVI）。为了比较它们并指导决定使用哪种GEVI，我们并排表征了代表不同分子结构家族的8种GEVI的性能。我们在体外用单光子成像测试GEVI，在体内用单光子宽场成像和双光子成像测试GEVI。我们发现，QuasAr 2在体外表现出最好的性能，而只有ArcLight-MT可以用来可靠地检测体内的电活动与2光子激发。没有一个单一的GEVI是理想的每一个实验。这些结果提供了一个指导，选择最佳的GEVI的具体应用。
Imaging voltage using fluorescent-based sensors could be an ideal technique to probe neural circuits with high spatiotemporal resolution. However, due to insufficient signal-to-noise ratio (SNR), imaging membrane potential in mammalian preparations is still challenging. In recent years, many genetically encoded voltage indicators (GEVIs) have been developed. To compare them and guide decisions on which GEVI to use, we have characterized side by side the performance of eight GEVIs that represent different families of molecular constructs. We tested GEVIs in vitro with 1-photon imaging and in vivo with 1-photon wide-field imaging and 2-photon imaging. We find that QuasAr2 exhibited the best performance in vitro, whereas only ArcLight-MT could be used to reliably detect electrical activity in vivo with 2-photon excitation. No single GEVI was ideal for every experiment. These results provide a guide for choosing optimal GEVIs for specific applications.