RHO, RAC, AND CDC42 GTPASES REGULATE THE ASSEMBLY OF MULTIMOLECULAR FOCAL COMPLEXES ASSOCIATED WITH ACTIN STRESS FIBERS, LAMELLIPODIA, AND FILOPODIA
RHO, RAC, AND CDC42 GTPASES REGULATE THE ASSEMBLY OF MULTIMOLECULAR FOCAL COMPLEXES ASSOCIATED WITH ACTIN STRESS FIBERS, LAMELLIPODIA, AND FILOPODIA
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DOI：
10.1016/0092-8674(95)90370-4
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发表时间：
1995-04-07
期刊：
影响因子：
64.5
通讯作者：
HALL, A
中科院分区：
文献类型：
--
作者：
NOBES, CD;HALL, A
Rho和rac是ras相关的小GTP酶超家族的两个成员，分别调节肌动蛋白的聚合以产生应力纤维和板状伪足。我们在这里报告，cdc42，rho家族的另一个成员，触发了细胞外围第三种肌动蛋白结构的形成，丝状伪足。除了应力纤维，rho还控制粘着斑复合物的组装。我们现在发现rac和cdc42也刺激质膜上多分子焦点复合物的组装。这些复合物，这是与板状伪足和丝状伪足，包含黏着斑蛋白，桩蛋白，和黏着斑激酶，但不同的，并形成独立的rho诱导的黏着斑。瑞士3T3细胞中cdc42的激活导致rac和rho的顺序激活，这表明由GTP酶的rho家族成员协调控制细胞运动的分子模型。
Rho and rac, two members of the ras-related superfamily of small GTPases, regulate the polymerization of actin to produce stress fibers and lamellipodia, respectively. We report here that cdc42, another member of the rho family, triggers the formation of a third type of actin-based structure found at the cell periphery, filopodia. In addition to stress fibers, rho controls the assembly of focal adhesion complexes. We now show that rac and cdc42 also stimulate the assembly of multimolecular focal complexes at the plasma membrane. These complexes, which are associated with lamellipodia and filopodia, contain vinculin, paxillin, and focal adhesion kinase, but are distinct from and formed independently of rho-induced focal adhesions. Activation of cdc42 in Swiss 3T3 cells leads to the sequential activation of rac and then rho, suggesting a molecular model for the coordinated control of cell motility by members of the rho family of GTPases.