Housing and testing in mixed-sex rooms increases motivation and accuracy during operant testing in both male and female mice
Housing and testing in mixed-sex rooms increases motivation and accuracy during operant testing in both male and female mice
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DOI：
10.1016/j.nlm.2018.01.005
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发表时间：
2018-04-01
影响因子：
2.7
通讯作者：
Reyes, Teresa M.
中科院分区：
文献类型：
--
作者：
Lloyd, Kelsey R.;Yaghoubi, Sarah K.;Reyes, Teresa M.
操作行为任务广泛应用于神经科学研究，但很少有人知道如何变量，如住房和测试条件影响啮齿动物的操作性能。我们之前已经观察到雄性和雌性小鼠的操作性能差异，这取决于小鼠是否在仅包含一种性别的房间与包含两种性别的房间中饲养和测试。在这里，雄性和雌性小鼠在单一性别或混合性别的住房房间进行了训练，固定比例1（FR1）和渐进比例（PR）的任务。对于两种性别，混合性别室中的动物在FR 1中的表现更准确，并且在PR任务中更有动力。然后，我们将单性别圈养动物移至混合性别室，反之亦然。开始在混合性别饲养的动物PR没有变化，但开始在单一性别饲养的两种性别在转换后都更有动力。此外，搬进单一性别住房的女性在FR 1中的表现不太准确。我们的结论是，住房和测试条件可以影响性能FR1和PR任务。由于这些任务通常用作更复杂任务的培训步骤，因此在实验设计期间应仔细考虑饲养和测试条件，并在出版物中报告。
Operant behavior tasks are widely used in neuroscience research, but little is known about how variables such as housing and testing conditions affect rodent operant performance. We have previously observed differences in operant performance in male and female mice depending on whether mice were housed and tested in rooms containing only one sex versus rooms containing both sexes. Here, male and female mice in either single-sex or mixed sex housing rooms were trained on fixed ratio 1 (FR1) and progressive ratio (PR) tasks. For both sexes, animals in the mixed sex room had more accurate performance in FR1 and were more motivated in the PR task. We then moved the single sex housed animals to the mixed sex room and vice versa. Animals that started in mixed sex housing had no change to PR, but both sexes who started in single sex housing were more motivated after the switch. Additionally, the females that moved into single-sex housing performed less accurately in FR1. We conclude that housing and testing conditions can affect performance on FR1 and PR tasks. As these tasks are commonly used as training steps to more complex tasks, housing and testing conditions should be carefully considered during experiment design and reported in publications.