3D trajectory of minute object by parallel phase-shifting digital holographic microscope
3D trajectory of minute object by parallel phase-shifting digital holographic microscope
复制标题
通过平行相移数字全息显微镜观察微小物体的 3D 轨迹
DOI：
10.1117/12.2573722
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Matoba Osamu
中科院分区：
文献类型：
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作者：
Awatsuji Yasuhiro;Inamoto Junya;Fukuda Takahito;Inoue Tomoyoshi;Matoba Osamu
本文综述了用平行移相数字全息技术测量微小物体的三维轨迹。平行移相数字全息术是一种能够单次记录动态物体波的复杂振幅分布的技术。作者构建了一种基于平行移相数字全息的倒置显微镜。该显微镜由连续波激光器、马赫-曾德尔干涉仪、偏振成像相机和放大光学系统组成。采用高速偏振成像摄像机记录动态试样全息图的运动图像。显微镜以每秒60帧的速度记录了一分钟明矾晶体在明矾溶液中下沉的全息图。在整个下沉时间内，成功地获得了晶体的重新聚焦图像。晶体的三维轨迹是由重新聚焦的图像导出的。构建了一种基于平行移相数字全息技术的倒置垂直显微镜。用显微镜以1000 FPS的速度记录了藻藻作为活微生物在水中游动的过程。利用重建图像成功地展示了微生物在500 μm × 500 μm × 500 μm区域内弯曲运动的三维轨迹。
The authors review three-dimensional (3D) trajectory of minute object by parallel phase-shifting digital holography. Parallel phase-shifting digital holography is a technique capable of single-shot recording of a complex amplitude distribution of object wave from a dynamic object. The authors constructed an inverted microscope based on parallel phase-shifting digital holography. The microscope consisted of a continuous-wave laser, a Mach–Zehnder interferometer, a polarization imaging camera, and a magnification optical system. A high-speed polarization imaging camera was employed to record motion picture of holograms of the dynamic specimen. Motion picture of the holograms of a minute alum crystal sinking down in the solution of alum was recorded by the microscope at the rate of 60 frames per seconds (FPS). Refocused images of the crystal were successfully obtained for all of the sinking time. The 3D trajectory of the crystal was derived from the refocused images. Also, the authors constructed an inverted and vertical microscope based on parallel phase-shifting digital holography. A Volvox swimming in a water as a living microbe was recorded by the microscope at the rate of 1000 FPS. The 3D trajectory of the microbe curvedly moving in the area of 500 μm × 500 μm × 500 μm was successfully demonstrated from the reconstructed images of the microbe.