MinION nanopore sequencing of an influenza genome.
MinION nanopore sequencing of an influenza genome.
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DOI：
10.3389/fmicb.2015.00766
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发表时间：
2015
影响因子：
5.2
通讯作者：
Hall RJ
中科院分区：
文献类型：
--
作者：
Wang J;Moore NE;Deng YM;Eccles DA;Hall RJ
流感流行和大流行对世界各地的经济、发病率和死亡率都有重大影响。快速准确地对流感病毒进行测序的能力对预防和缓解流感具有重要作用。用聚合酶链式反应同时扩增了甲型流感病毒的全部8个流感基因，然后在Minion纳米孔测序仪上进行了测序。获得了一个完整的流感病毒基因组，与Illumina MiSeq和传统的Sanger-Sanger测序获得的序列数据有99%以上的一致性。用于在Minion纳米孔测序仪上进行这项实验的实验室基础设施和计算资源将在世界各地的大多数分子实验室中使用。使用该系统，可携带性的概念，从而在临床或现场对流感病毒进行测序现在是成立的。
Influenza epidemics and pandemics have significant impacts on economies, morbidity and mortality worldwide. The ability to rapidly and accurately sequence influenza viruses is instrumental in the prevention and mitigation of influenza. All eight influenza genes from an influenza A virus were amplified by PCR simultaneously and then subjected to sequencing on a MinION nanopore sequencer. A complete influenza virus genome was obtained that shared greater than 99% identity with sequence data obtained from Illumina MiSeq and traditional Sanger-sequencing. The laboratory infrastructure and computing resources used to perform this experiment on the MinION nanopore sequencer would be available in most molecular laboratories around the world. Using this system, the concept of portability, and thus sequencing influenza viruses in the clinic or field is now tenable.