Comparison of the replication and persistence of simian-human immunodeficiency viruses expressing Vif proteins with mutation of the SLQYLA or HCCH domains in macaques.
Comparison of the replication and persistence of simian-human immunodeficiency viruses expressing Vif proteins with mutation of the SLQYLA or HCCH domains in macaques.
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DOI：
10.1016/j.virol.2010.04.017
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发表时间：
2010-09-01
期刊：
影响因子：
3.7
通讯作者：
Stephens EB
中科院分区：
文献类型：
--
作者：
Schmitt K;Hill MS;Liu Z;Ruiz A;Culley N;Pinson DM;Stephens EB
The Vif protein of primate lentiviruses interacts with APOBEC3 proteins, which results in shunting of the APOBEC3-Vif complex to the proteosome for degradation. Using the simian-human immunodeficiency virus (SHIV)/macaque model, we compared the replication and pathogenicity of SHIVs that express a Vif protein in which the entire SLQ(Y/F)LA (SHIVVif5A) or HCCH (SHIVVifHCCH(−)) domains were substituted with alanine residues. Each virus was inoculated into three macaques and various viral and immunological parameters followed for six months. All macaques maintained stable circulating CD4+ T cells, developed low viral loads, maintained the engineered mutations, yielded no histological lesions, and developed immunoprecipitating antibodies early post-inoculation. Sequence analysis of nef and vpu from three lymphoid tissues revealed a high percentage of G-to-A-substitutions. Our results show that while the presence of HCCH and SLQ(Y/F)LA domains are critical in vivo, there may exist APOBEC3 negative reservoirs that allow for low levels of viral replication and persistence but not disease.
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影响因子：
3.7
作者：
Hill, M. Sarah;Ruiz, Autumn;Stephens, Edward B.
通讯作者：
Stephens, Edward B.
影响因子：
64.5
作者：
Harris, RS;Bishop, KN;Malim, MH
通讯作者：
Malim, MH
影响因子：
4.8
作者：
Mehle, A;Strack, B;Gabuzda, D
通讯作者：
Gabuzda, D
影响因子：
4.8
作者：
Dang, Ying;Siew, Lai Mun;Zheng, Yong-Hui
通讯作者：
Zheng, Yong-Hui
影响因子：
1.5
作者：
Hill, M. Sarah;Mulcahy, Ellyn R.;Stephens, Edward B.
通讯作者：
Stephens, Edward B.