Amphotericin B loaded SLN prepared with the coacervation technique
Amphotericin B loaded SLN prepared with the coacervation technique
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DOI：
10.1016/s1773-2247(10)50028-5
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发表时间：
2010-05-01
影响因子：
5
通讯作者：
Battaglia, L.
中科院分区：
文献类型：
--
作者：
Bianco, M. A.;Gallarate, M.;Battaglia, L.
采用凝聚法制备了硬脂酸固体脂质纳米粒(SLN)。SLN可以高效地以单体形式包封两性霉素B。SLN对酿酒酵母(模型酵母)的体外药物毒性显著增加，表现为钾渗漏。与游离药物相比，当药物负载到纳米粒中时，酵母对两性霉素B的敏感性增加，即使抑制受到脂质基质中药物包裹的限制。
Stearic acid solid lipid nanoparticles (SLN) were prepared according to the coacervation technique, consisting fatty acid precipitation of alkaline salt from a solution. SLN can encapsulate Amphotericin B with high efficiency in its monomeric form. SLN markedly increase in vitro drug toxicity towards Saccharomyces cerevisiae (taken as model yeast), expressed as potassium leakage. Yeast susceptibility towards Amphotericin B is increased when the drug is loaded in nanoparticles compared to the free drug, even if inhibition is limited by drug encapsulation within lipid matrix..