A single β adaptin contributes to AP1 and AP2 complexes and clathrin function in Dictyostelium.
A single β adaptin contributes to AP1 and AP2 complexes and clathrin function in Dictyostelium.
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DOI：
10.1111/j.1600-0854.2011.01310.x
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发表时间：
2012-02
期刊：
影响因子：
--
通讯作者：
O'Halloran TJ
中科院分区：
文献类型：
--
作者：
Sosa RT;Weber MM;Wen Y;O'Halloran TJ
网格蛋白包被囊泡的组装对许多细胞过程都很重要，包括营养摄取和膜组织。网格蛋白组装的重要贡献者是四种四聚体组装蛋白，也称为接头蛋白（AP），每一种都含有一个β亚基。我们发现了一个单一的β亚基，命名为β1/2，它对盘基骨菌的AP1和AP2复合物都有贡献。编码β1/2基因的破坏导致生长、细胞分裂和发育的严重缺陷。此外，缺乏β1/2的细胞表现出严重的渗透调节缺陷，包括缺乏收缩液泡和收缩液泡标记物的错误定位。β1/2的表型与先前描述的网格蛋白和AP1突变体的表型最相似，这支持AP1在盘形骨鞘细胞中对网格蛋白通路的特别重要贡献。细胞中β1/2的缺失导致AP1和AP2复合体的中等亚基的蛋白量显著减少，从而确立了β亚基在中等亚基稳定性中的作用。盘基骨柱β1/2可能类似于脊椎动物AP复合物中更特化的β1和β2亚基的共同祖先。我们的结果支持单个β亚基对简单真核生物AP1和AP2的稳定性和功能的重要贡献。
The assembly of clathrin-coated vesicles is important for numerous cellular processes, including nutrient uptake and membrane organization. Important contributors to clathrin assembly are four tetrameric Assembly Proteins, also called Adaptor Proteins (AP’s), each of which contains a beta subunit. We identified a single beta subunit, named β1/2, that contributes to both the AP1 and AP2 complexes of Dictyostelium. Disruption of the gene encoding β1/2 resulted in severe defects in growth, cytokinesis, and development. Additionally, cells lacking β1/2 displayed profound osmoregulatory defects including the absence of contractile vacuoles and mislocalization of contractile vacuole markers. The phenotypes of β1/2 were most similar to previously described phenotypes of clathrin and AP1 mutants, supporting a particularly important contribution of AP1 to clathrin pathways in Dictyostelium cells. The absence of β1/2 in cells led to significant reductions in the protein amounts of the medium-sized subunits of the AP1 and AP2 complexes, establishing a role for the beta subunit in the stability of the medium subunits. Dictyostelium β1/2 could resemble a common ancestor of the more specialized β1 and β2 subunits of the vertebrate AP complexes. Our results support the essential contribution a single beta subunit to the stability and function AP1 and AP2 in a simple eukaryote.
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DOI：
10.1083/jcb.121.6.1311
发表时间：
1993-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Heuser J;Zhu Q;Clarke M
通讯作者：
Clarke M
影响因子：
56.9
作者：
Dell'Angelica, EC;Klumperman, J;Bonifacino, JS
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Bonifacino, JS
影响因子：
11.4
作者：
Meyer, C;Zizioli, D;Schu, P
通讯作者：
Schu, P
DOI：
10.1073/pnas.86.8.2612
发表时间：
1989-04-01
影响因子：
11.1
作者：
KIRCHHAUSEN, T;NATHANSON, KL;DAVIS, AE
通讯作者：
DAVIS, AE
DOI：
10.1073/pnas.0707318105
发表时间：
2008-01-15
影响因子：
11.1
作者：
Dacks, Joel B.;Poon, Pak P.;Field, Mark C.
通讯作者：
Field, Mark C.