Characteristics and phylogenetic analysis of the facultative anaerobic dissimilatory azoreducing bacteria from activated sludge
Characteristics and phylogenetic analysis of the facultative anaerobic dissimilatory azoreducing bacteria from activated sludge
复制标题
活性污泥兼性厌氧异化偶氮还原菌的特性及系统发育分析
DOI：
10.1016/j.ibiod.2007.10.007
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发表时间：
2008-06-01
影响因子：
4.8
通讯作者：
Sun, Guoping
中科院分区：
文献类型：
--
作者：
Hong, Yiguo;Guo, Jun;Sun, Guoping
分离了生理上不同的兼性厌氧微生物，并研究了它们以偶氮化合物作为终端电子受体氧化不同底物的能力。四株异化的azoreducing细菌(出众的人或物),隔绝纺织印染污水处理厂的活性污泥,可以降低耦合的氧化偶氮的化合物的电子给体。不同菌株对偶氮还原的电子供体有不同的偏好，如氢、甲酸盐或乳酸盐。通过16S rDNA序列的系统发育分析，研究了这些darb之间的进化关系。Citrobacter属（AzoR-1）、Acinetobacter属（Azc R-3）和Pseudomonas属（Azo R-9）的成员组成了变形菌纲γ亚群中的单系群，与之前描述的通过异化偶氮还原过程获得生长能量的Sheivanella脱色菌S12成员密切相关。芽孢杆菌属（Azo R-6）在厚壁菌门中形成了一个独特的分支。本研究的结果扩大了已知能够异化偶氮还原的有限数量的微生物分离株，并证明了偶氮还原与氢或有机酸作为电子供体偶联的普遍性。(C) 2007年Elsevier Ltd.出版
Physiologically distinct facultative anaerobic microorganisms were isolated and investigated for their ability to oxidize different substrates with azo compounds as a terminal electron acceptor. Four strains of dissimilatory azoreducing bacteria (DARBs), isolated from activated sludge of a textile-printing wastewater treatment plant, could reduce azo compound by coupling oxidation of several of electron donors. Different strains preferred specific electron donor for azoreduction, such as hydrogen, formate or lactate. Evolutionary relationships among these DARBs were examined by phylogenetic analysis of 16S rDNA sequences. Members of the genera Citrobacter (AzoR-1), Acinetobacter(Azc R-3), and Pseudomonas(Azo R-9) formed a monophyletic group within the gamma subdivision of the class Proteobacteria, which was closely related to the member of the previously described Sheivanella decolorationnis S12 that obtained its energy for growth by dissimilatory azoreduction process. The genus Bacillus (Azo R-6) made up a distinct branch within the Firmicutes cluster. The results of this study expanded the limited number of microbial isolates that are known to be capable of dissimilatory azoreduction and demonstrated that the ubiquity of azoreduction coupling with hydrogen or organic acids as an electron donor. (C) 2007 Published by Elsevier Ltd.