Direct observation of ligand recognition by T cells
Direct observation of ligand recognition by T cells
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DOI：
10.1038/nature01076
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发表时间：
2002-10-24
期刊：
影响因子：
64.8
通讯作者：
Davis, MM
中科院分区：
文献类型：
--
作者：
Irvine, DJ;Purbhoo, MA;Davis, MM
通过T细胞受体与抗原呈递细胞（APC）表面的主要组织相容性复合物（MHC）结合的抗原肽相互作用来激活T细胞是获得性免疫的关键步骤。在这里，我们使用三维荧光显微镜观察单个肽-I-E-k II类MHC复合物标记的藻胆蛋白藻红蛋白，努力表征T细胞的敏感性和在单细胞中形成免疫突触的要求（1-3）。我们表明，表达CD 4抗原的T细胞响应瞬时钙信号，即使是一个单一的激动剂肽-MHC配体，和组织的分子在T细胞和APC的接触区的免疫突触的特征时，只有约10激动剂存在。这种敏感性高度依赖于CD 4，因为用抗体阻断这种分子会使T细胞无法检测到少于约30种配体。
The activation of T cells through interaction of their T-cell receptors with antigenic peptide bound to major histocompatibility complex (MHC) on the surface of antigen presenting cells (APCs) is a crucial step in adaptive immunity. Here we use three-dimensional fluorescence microscopy to visualize individual peptide-I-E-k class II MHC complexes labelled with the phycobiliprotein phycoerythrin in an effort to characterize T-cell sensitivity and the requirements for forming an immunological synapse(1-3) in single cells. We show that T cells expressing the CD4 antigen respond with transient calcium signalling to even a single agonist peptide-MHC ligand, and that the organization of molecules in the contact zone of the T cell and APC takes on the characteristics of an immunological synapse when only about ten agonists are present. This sensitivity is highly dependant on CD4, because blocking this molecule with antibodies renders T cells unable to detect less than about 30 ligands.