Evaluating the Photodynamic Biocidal Activity and Investigating the Mechanism of Thiazolium Cyanine Dyes
Evaluating the Photodynamic Biocidal Activity and Investigating the Mechanism of Thiazolium Cyanine Dyes
复制标题
噻唑花青染料光动力杀菌活性评价及机理研究
DOI：
10.1021/acsabm.9b01182
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发表时间：
2020
影响因子：
4.7
通讯作者：
Liao Wei
中科院分区：
文献类型：
--
作者：
Wang Jing;Zhao Peng;Li Xiangyu;Fu Huaxia;Yang Xia;Wang Guanquan;Yang Yuchuan;Wei Hongyuan;Zhou Zhijun;Liao Wei
为了开发理想的光动力疗法(PDT)光敏剂，在菁染料中引入了噻唑基，使其具有可见光强吸收和抗微生物活性的优点。我们评价了三种噻唑菁染料对金黄色葡萄球菌(革兰氏阳性菌)和大肠杆菌(革兰氏阴性菌)的体外抗菌活性。这是此类菁染料首次应用于光动力抗生素领域。在白光照射下，花青素对金黄色葡萄球菌和大肠杆菌表现出良好的抑菌活性，相应的光诱导杀菌效果随照射时间和浓度的增加而增加。同时，也没有观察到暗抗菌能力。尤其是花菁具有较低的细胞毒性和良好的生物相容性。这些结果表明，四氮杂菁作为光敏剂在光动力疗法中具有广阔的应用前景和杀菌前景。机理研究表明，活性氧(ROS)是对抗白光环境下细菌的重要因素，但不是唯一决定杀菌活性的因素。花菁与细胞膜的相互作用在杀菌过程中也起着关键作用，表现出静电和疏水相互作用的协同效应。它影响细胞对花菁的摄取和膜的扰动活性，从而间接影响生物杀灭活性。
In order to develop ideal photosensitizers for photodynamic therapy (PDT), a thiazolium group was introduced in cyanine dyes to possess the advantages of intense absorption in the visible region and anti-microbial activity. We evaluated the anti-bacterial activity of the three thiazolium cyanine dyes against Staphylococcus aureus (Gram-positive bacteria) and Escherichia coli (Gram-negative bacteria) in vitro. This is the first time that such kinds of cyanine dyes are applied in the photodynamic antibiotic area. Cyanines display excellent anti-bacterial activity to S. aureus and E. coli exposed to white light irradiation, and the corresponding light-induced biocidal efficacy of cyanines increased with irradiation time and concentration. At the same time, there was no observation of dark anti-bacterial abilities. Especially, cyanines show low cell cytotoxicity and excellent biocompatibility. These results suggest that thiazolium cyanine could work as a photosensitizer in PDT with great promise and broad applications for killing bacteria. Mechanistic studies suggest that the reactive oxygen species (ROS) is the vital factor for combating bacteria exposure to white light conditions, whereas it is not the only determining factor of the biocidal activity. The interaction of the cyanine to the cell membrane also plays a critical role in killing bacteria, which has exhibited a synergic effect of electrostatic and hydrophobic interactions. It influences the cell uptake and the membrane perturbation activity of the cyanines, which indirectly affects the biocidal activity.