A new model of skeletal muscle atrophy induced by immobilization using a hook-and-loop fastener in mice.
A new model of skeletal muscle atrophy induced by immobilization using a hook-and-loop fastener in mice.
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DOI：
10.1589/jpts.29.1779
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发表时间：
2017-10
影响因子：
--
通讯作者：
Hagiwara H
中科院分区：
文献类型：
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作者：
Aihara M;Hirose N;Katsuta W;Saito F;Maruyama H;Hagiwara H
【目的】为了研究肌肉萎缩，肌萎缩模型小鼠已被广泛使用。特别地，石膏固定是诱导肌肉萎缩最常见的方法。然而，它是耗时的，并经常引起不良事件，包括皮肤损伤，水肿和坏死。在本研究中，我们开发了一种新的、简单的、侵入性较小的诱导肌肉萎缩的钩环固定方法（魔术贴）。【对象与方法】小鼠膝关节伸展和踝关节足底伸展体位包扎。肌肉萎缩是由在肢体上缠绕石膏或魔术贴引起的。[结果]根据体重和纤维大小，Velcro固定诱导等量肌肉萎缩。尼龙搭扣固定显著减少了手术时间和不良事件的发生频率。[结论]与石膏固定相比，尼龙搭扣固定可引起肌肉萎缩，但时间短，并发症少。维可牢固定在物理治疗小鼠模型的临床实践中可能有助于废用性肌萎缩的研究。
[Purpose] To study muscle atrophy, the muscle atrophy model mice have been used frequently. In particular, cast immobilization is the most common method to induce muscle atrophy. However, it is time consuming and often causes adverse events including skin injury, edema, and necrosis. The present study, we developed a hook-and-loop fastener (Velcro) immobilization method as a new, simple, and less invasive approach to induce muscle atrophy. [Subjects and Methods] Mice were bandaged in the knee joint extension and ankle plantar extension position. Muscle atrophy was induced by either winding a cast or Velcro around the limb. [Results] According to weight and fiber size, Velcro immobilization induced equivalent muscle atrophy to cast immobilization. Velcro immobilization reduced significantly the time for the procedure and the frequency of adverse events. [Conclusion] Velcro immobilization can induce muscle atrophy comparable to cast immobilization, but in a shorter time and with less complications. Velcro immobilization may contribute to the study of disuse muscle atrophy in clinical practice of physical therapy using a mouse model.