Nucleotide sequence of the gene encoding NADH dehydrogenase from an alkalophile, Bacillus sp. strain YN-1.
Nucleotide sequence of the gene encoding NADH dehydrogenase from an alkalophile, Bacillus sp. strain YN-1.
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编码来自嗜碱性芽孢杆菌属的 NADH 脱氢酶的基因的核苷酸序列。
DOI：
10.1093/oxfordjournals.jbchem.a123440
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发表时间：
1991
影响因子：
2.7
通讯作者：
Tairo Oshima
中科院分区：
文献类型：
--
作者：
Xuemin Xu;Noriyuki Koyama;M. Cui;Akihiko Yamagishi;Yoshiaki Nosoh;Tairo Oshima
对来自嗜碱菌芽孢杆菌属的编码 NADH 脱氢酶的基因进行了克隆和测序。克隆的DNA片段含有1,557个核苷酸的开放阅读框，编码由519个氨基酸残基组成的多肽(Mr 55,830)。预测的氨基酸序列与前期研究确定的部分氨基酸序列（包括N端和C端序列）一致。与其他黄素酶的序列比较表明本脱氢酶与大肠杆菌硫氧还蛋白还原酶具有高度同源性。
The gene encoding NADH dehydrogenase from an alkalophile, Bacillus sp., was cloned and sequenced. The cloned DNA fragment contained an open reading frame of 1,557 nucleotides which encodes a polypeptide composed of 519 amino acid residues (Mr 55,830). The predicted amino acid sequence was consistent with the partial amino acid sequences including the N-terminal and C-terminal sequences determined in a previous study. Sequence comparison with other flavoenzymes revealed high homology between the present dehydrogenase and Escherichia coli thioredoxin reductase.