Detection of anammox activity and diversity of anammox bacteria-related 16S rRNA genes in coastal marine sediment in japan
Detection of anammox activity and diversity of anammox bacteria-related 16S rRNA genes in coastal marine sediment in japan
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DOI：
10.1264/jsme2.22.232
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发表时间：
2007-01-01
影响因子：
2.2
通讯作者：
Suwa, Yuichi
中科院分区：
文献类型：
--
作者：
Amano, Teruki;Yoshinaga, Ikuo;Suwa, Yuichi
使用 N-15 示踪剂对日本沿海海洋沉积物样本进行了厌氧氨氧化活动（一种独特的 N-2 排放过程）的首次评估。还通过对已知厌氧氨氧化细菌的 16S rRNA 基因进行选择性 PCR 扩增，评估了可能与厌氧氨氧化过程有关的细菌的出现和多样性。通过测量 (NN)-N-14-N-15 气体产量来检测厌氧氨氧化活性，仅在位于淀川河口的潮间带沙洲采集的样品中发现。在淀河样本中，还发现了属于已知厌氧氨氧化细菌谱系的16S rRNA基因片段，并且两个主要系统型均为“Candidatus Scalindua wagneri”近缘，具有95%和98%的序列相似性。即使在未检测到可识别的厌氧氨氧化活性的其他样本中，也检测到与已知厌氧氨氧化细菌相关的16S rRNA基因片段，但与“Ca. S. wagneri”无关。这是日本沿海海洋环境中厌氧氨氧化介导的 N-2 排放的首次报告。值得注意的是，基于 PCR 的分析使我们能够发现厌氧氨氧化细菌相关 16S rRNA 基因序列的意外系统发育多样性。本研究中开发的选择性 PCR 引物组可以成为揭示自然环境中厌氧氨氧化细菌生态学的有力工具。
A first assessment of anammox activity, which is a unique N-2 emission process, was conducted in samples of coastal marine sediment from Japan with a N-15 tracer. The occurrence and diversity of bacteria possibly responsible for the anammox process were also evaluated by selective PCR-amplification of the 16S rRNA gene for known anammox bacteria. Anammox activity, detected by measuring (NN)-N-14-N-15 gas production, was only found in samples collected at the intertidal sand bank located at the Yodo River estuary. In the Yodo River samples, 16S rRNA gene fragments affiliated with the known anammox bacterial lineage were also recovered, and the two major phylotypes were both "Candidatus Scalindua wagneri" relatives with 95% and 98% sequence similarity. Even from the other samples in which no recognizable anammox activity was detected, 16S rRNA gene fragments related to known anammox bacteria, but not to "Ca. S. wagneri", were detected. This is the first report of anammox-mediated N-2 emission in coastal marine environments in Japan. Notably, the PCR-based analysis allowed us to discover unexpected phylogenetic diversity of anammox bacteria-related 16S rRNA gene sequences. The selective PCR primer set developed in this study could be a powerful tool to unveil the ecology of anammox bacteria in natural environments.