Key role of alkaline phosphatase in the development of human-derived nanoparticles in vitro
Key role of alkaline phosphatase in the development of human-derived nanoparticles in vitro
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DOI：
10.1016/j.actbio.2010.10.027
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发表时间：
2011-03-01
影响因子：
9.7
通讯作者：
Lieske, John C.
中科院分区：
文献类型：
--
作者：
Hunter, Larry W.;Shiekh, Farooq A.;Lieske, John C.
碱性磷酸酶(ALP)是一种生理和病理生物矿化的关键酶。实验旨在确定碱性磷酸酶是否参与体外钙化纳米颗粒(NPs)的形成。将过滤后的人颈动脉、主动脉和肾结石的匀浆接种到含10%胎牛血清的细胞培养液中，不加或不加碱性磷酸酶或焦磷酸盐的抑制剂。接种后1周内形成钙化性NP生物膜，焦磷酸盐和碱性磷酸酶抑制剂可抑制其形成。无论是钙化的还是脱钙的，洗涤后的NPs中都检测到了碱性磷酸酶蛋白和酶活性。因此，正如先前在体内病理性钙化过程中所涉及的那样，体外钙化NPs的形成需要碱性磷酸酶的活性。(C)2010年Acta Materialia Inc.由爱思唯尔有限公司出版。版权所有。
Alkaline phosphatase (ALP) is an enzyme critical for physiological and pathological biomineralization. Experiments were designed to determine whether ALP participates in the formation of calcifying nanometer sized particles (NPs) in vitro. Filtered homogenates of human calcified carotid artery, aorta and kidney stones were inoculated into cell culture medium containing 10% fetal bovine serum in the absence or presence of inhibitors of ALP or pyrophosphate. A calcific NP biofilm developed within 1 week after inoculation and their development was reduced by pyrophosphate and inhibitors of ALP. ALP protein and enzymatic activity were detected in washed NPs, whether calcified or decalcified. Therefore, ALP activity is required for the formation of calcifying NPs in vitro, as has previously been implicated during pathological calcification in vivo. (C) 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.