Cardiotonic effect of Apocynum venetum L. extracts on isolated guinea pig atrium
Cardiotonic effect of Apocynum venetum L. extracts on isolated guinea pig atrium
复制标题
DOI：
10.1007/s11418-008-0296-2
复制
发表时间：
2009-04-01
影响因子：
3.3
通讯作者：
Himi, Toshiyuki
中科院分区：
文献类型：
--
作者：
Irie, Kaoru;Sato, Takahiro;Himi, Toshiyuki
通过记录豚鼠右心房机械活动和心率，研究罗布麻叶、根、茎、老茎和富含多酚的叶提取物--罗布麻提取物对豚鼠心肌的影响。还用LC-MS/MS分析了脉络草提取物中的半胱氨酸--一种强心苷。所有提取物均表现出较弱的强心作用，即在浓度为1 mg/m L时引起离体心房收缩反应和脉搏增加，而β-肾上腺素能受体阻滞剂心得安(1 MU M)对此无抑制作用。木犀草素的含量大小顺序为：老茎；茎；根；叶；由于紫云英提取物的强心作用不能反映半胱氨酸的含量，因此探讨了除强心苷外的另一种可能机制。用无细胞酶方法研究了其对磷酸二酯酶3(PDE3)的抑制作用。这些结果提示，抑制PDE3可能参与了蛇床子草提取物的强心作用。
The effects on guinea-pig heart muscle of extracts of Apocynum venetum L. leaf, root, stem, old stem and Venetron-a polyphenol-rich extract of leaves-were studied by recording the mechanical activity and heart rate of isolated right atria. Cymarin-a cardiac glycoside-was also determined in A. venetum extracts by LC-MS/MS analysis. All extracts examined here showed a weak cardiotonic effect, i.e., induced a contractile response of the isolated atria and increased the pulse at a concentration of 1 mg/mL, which was not inhibited by propranolol (1 mu M)-a beta-adrenoceptor blocker. The cymarin content in extracts of A. venetum was ranked as follows: old stem >> stem > root > leaf >> Venetron. Since the cardiotonic effects of A. venetum extracts did not reflect the cymarin content, a possible mechanism other than that of cardiac glycosides was investigated. The inhibitory effects on phosphodiesterase 3 (PDE3) were studied in a cell-free enzyme assay; all extracts of various parts of A. venetum inhibited PDE purified from human platelets. These results suggest that PDE3 inhibition may contribute to the cardiotonic effects of A. venetum extracts.