The effects of Kloeckera apiculata on the cuticular waxes of navel orange fruit
The effects of Kloeckera apiculata on the cuticular waxes of navel orange fruit
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Kloeckera apiculata 对脐橙果实角质层蜡质的影响
DOI：
10.1016/j.biocontrol.2014.03.006
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发表时间：
2014-06-01
影响因子：
4.2
通讯作者：
Long, Chao-an
中科院分区：
文献类型：
--
作者：
Liu, Pu;Hao, Hao-hao;Long, Chao-an
从根际土壤中选育出防治柑桔青霉病和青霉病效果较好的34-9菌株。以成熟的纽荷尔脐橙为试材，研究了拮抗酵母菌K.apulata对柑橘角质蜡的影响。我们的研究结果表明，刺梨能促进果皮蜡质的产生和果实表面蜡质形态的变化。利用Affymetrix柑橘基因组芯片鉴定了15个柑橘蜡质代谢相关差异表达基因(DEG)。采用GC-MS联用技术，从纽荷尔果实表面分离出46种蜡质化合物。一方面，在处理条件下，包括二十二酸、1，2，3-丙三酯和9-十六烯酸、9-十八烯基酯在内的酯类化合物的表达水平比对照高近一倍；另一方面，脂肪酸和脂肪醇在处理条件下分别降低了74.4%和72.3%。处理后产生的新蜡和纽荷尔表面疏水性的增加可能会影响孢子的黏附和萌发。此外，果实蜡质对刺激物的反应可能受细胞内过氧化氢信号的调节。本研究揭示了纽荷尔脐橙果实对红曲霉的反应，从而为了解拮抗酵母菌在采后果实中的作用机制提供了新的线索。皇冠版权所有(C)2014由爱思唯尔公司出版。保留所有权利。
Kloeckera apiculata 34-9 was selected from the rhizosphere soil for its high efficacy in controlling citrus green and blue mold. In this study, the effect of the antagonistic yeast K. apiculata on citrus cuticular wax was investigated in ripening Newhall navel orange (Citrus sinensis L. Osbeck). Our results show that K. apiculata triggers the production of cuticular waxes and surface wax morphology changes in the fruit surface. 15 K. apiculata-responsive differentially expressed genes (DEGs) were identified for wax metabolism by using the Affymetrix citrus genome GeneChip. Using GC-MS, 46 wax compounds were found in the Newhall fruit surface. On one hand, esters including docosanoic acid, 1,2,3-propanetriyl ester and 9-hexadecenoic acid, 9-octadecenyl ester were up-regulated approximately twofold in the treatment condition compared with the control; and on the other hand, the fatty acids and fatty alcohols decreased by 74.4% and 72.3%, respectively, in the treatment condition. The new wax production and increased hydrophobicity of the Newhall surface resulting from the treatment may influence spore adhesion and germination. Furthermore, the response of the fruit waxes to the K. apiculata stimulus is likely to be regulated by intra-cellular H2O2 signaling. This study demonstrated the response fruit waxes to K. apiculata in Newhall navel oranges, thus providing new clues that aid our understanding of the mechanisms of action of antagonistic yeasts in postharvest fruits. Crown Copyright (C) 2014 Published by Elsevier Inc. All rights reserved.