MdHY5 positively regulates cold tolerance via CBF-dependent and CBF-independent pathways in apple
MdHY5 positively regulates cold tolerance via CBF-dependent and CBF-independent pathways in apple
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MdHY5 通过 CBF 依赖和 CBF 独立途径正向调节苹果的耐寒性
DOI：
10.1016/j.jplph.2017.09.001
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发表时间：
2017-11-01
影响因子：
4.3
通讯作者：
Hao, Yu-Jin
中科院分区：
文献类型：
--
作者：
An, Jian-Ping;Yao, Ji-Fang;Hao, Yu-Jin
冷胁迫是影响作物品质和产量的主要外部刺激因素。CBF冷调控途径一直被认为是冷应激反应的主要调控途径。在本研究中，我们发现苹果bZIP转录因子MdHY5在转录和翻译后水平上都对冷处理有响应。此外，MdHY5的过表达增强了苹果愈伤组织和拟南芥的耐寒性。随后，EMSA实验和瞬时表达实验表明，MdHY5通过结合MdCBF1启动子的G-Box基序，正向调节MdCBF1的转录。此外，MdHY5还能调控cbf不依赖的冷调控基因的表达。综上所述，我们的数据表明，MdHY5通过cbf依赖性和cbf非依赖性途径正向调节植物的耐寒性，这为MdHY5调控苹果的耐寒性提供了更深入的了解。
Cold stress is a major external stimulator that affects crop quality and productivity. The CBF cold regulatory pathway has been regarded as a master regulator in the response to cold stress. In this study, we found that the apple bZIP transcription factor, MdHY5, was responsive to cold treatment both at the transcriptional and at the post-translational levels. Moreover, overexpression of MdHY5 enhanced cold tolerance in apple calli and Arabidopsis. Subsequently, EMSA assay and transient expression assay demonstrated that MdHY5 positively regulated the transcript of MdCBF1 by binding to G-Box motif of its promoter. Furthermore, MdHY5 also regulated the expression of CBF-independent cold-regulated genes. Taken together, our data suggest that MdHY5 positively modulates plant cold tolerance through CBF-dependent and CBF-independent pathways, providing a deeper understanding of MdHY5-regulated cold tolerance in apple.