ELECTROSPRAY IONIZATION FOR MASS-SPECTROMETRY OF LARGE BIOMOLECULES
ELECTROSPRAY IONIZATION FOR MASS-SPECTROMETRY OF LARGE BIOMOLECULES
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DOI：
10.1126/science.2675315
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发表时间：
1989-10-06
期刊：
影响因子：
56.9
通讯作者：
WHITEHOUSE, CM
中科院分区：
文献类型：
--
作者：
FENN, JB;MANN, M;WHITEHOUSE, CM
电喷雾离子化技术是近年来发展起来的一种在真空中从溶液中的大而复杂的物质产生完整离子的强大技术。在某种程度上，这种技术比以前更熟悉的“软”电离方法更有可能，使质谱分析的力量和优雅适用于在生物系统中发挥重要作用的大而脆弱的极性分子。大分子的电喷雾光谱的显著特征是其组分离子是多电荷的峰的相干序列，每个峰的离子与序列中相邻相邻峰的离子相差一个电荷。光谱已经获得了生物聚合物，包括寡核苷酸和蛋白质，后者的分子量高达130，000，至今没有上限的证据。
Electrospray ionization has recently emerged as a powerful technique for producing intact ions in vacuo from large and complex species in solution. To an extent greater than has previously been possible with the more familiar "soft" ionization methods, this technique makes the power and elegance of mass spectrometric analysis applicable to the large and fragile polar molecules that play such vital roles in biological systems. The distinguishing features of electrospray spectra for large molecules are coherent sequences of peaks whose component ions are multiply charged, the ions of each peak differing by one charge from those of adjacent neighbors in the sequence. Spectra have been obtained for biopolymers including oligonucleotides and proteins, the latter having molecular weights up to 130,000, with as yet no evidence of an upper limit.