Measurement of feto-maternal haemorrhage: a comparative study of three Kleihauer techniques and tow flow cytometry methods.
Measurement of feto-maternal haemorrhage: a comparative study of three Kleihauer techniques and tow flow cytometry methods.
复制标题
胎儿-母体出血的测量：三种 Kleihauer 技术和双流式细胞术方法的比较研究。
DOI：
10.1046/j.1365-2257.1997.00216.x
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
JenniferKM Duguid
中科院分区：
文献类型：
--
作者：
I. Bromilow;JenniferKM Duguid
在英国，所有RhD阴性妇女在RhD阳性婴儿出生后常规进行Kleihauer试验，以确保给予足够剂量的抗D免疫球蛋白。解释Kleihauer试验结果以评估任何胎儿-母体出血量，必要时给予额外的抗D免疫球蛋白。当已知发生与同种异体免疫相关的事件时，产前也遵循类似的程序。Kleihauer试验的表现很难标准化，在解释胎儿-母体出血量时可能存在问题。据报道，使用流式细胞术测量胎儿-孕产妇出血可以提供更准确、更可靠的结果。本研究比较了三种不同的Kleihauer方法和两种不同的流式细胞术技术，所有这些方法都使用相同的母体样本，并在一个实验室内进行。结果证明了所用Kleihauer试验之间以及流式细胞术测量之间的变异性。一个良好的Kleihauer试验似乎仍然是有用的筛查技术检测胎儿-母体出血。然而，准确定量的大小胎儿-母体出血更可靠地确定流式细胞术。
In the UK a Kleihauer test is routinely performed on all RhD-negative women after the birth of an RhD-positive child to ensure that an adequate dose of anti-D immunoglobulin is given. The results of Kleihauer testing are interpreted to assess the volume of any feto-maternal haemorrhage and additional anti-D immunoglobulin is administered if necessary. A similar procedure is followed ante-natally when incidents occur known to be associated with alloimmunization. The performance of Kleihauer tests is difficult to standardize and there can be problems in interpreting the volume of feto-maternal haemorrhage. The use of flow cytometry to measure feto-maternal haemorrhage is reported to give more accurate and reliable results. This study compared three different Kleihauer methods and two different flow cytometry techniques all performed using the same maternal sample and within a single laboratory. The results demonstrated variability between the Kleihauer tests used and also in the flow cytometry measurements. A well-performed Kleihauer test would still appear to be useful as a screening technique for detection of feto-maternal haemorrhage. However, accurate quantitation of size of feto-maternal haemorrhage is more reliably determined by flow cytometry.