TRIPHENYLTETRAZOLIUM CHLORIDE (TTC) AS A MARKER FOR ISCHEMIC CHANGES IN RAT-BRAIN FOLLOWING PERMANENT MIDDLE CEREBRAL-ARTERY OCCLUSION
TRIPHENYLTETRAZOLIUM CHLORIDE (TTC) AS A MARKER FOR ISCHEMIC CHANGES IN RAT-BRAIN FOLLOWING PERMANENT MIDDLE CEREBRAL-ARTERY OCCLUSION
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DOI：
10.1111/j.1365-2990.1991.tb00694.x
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发表时间：
1991-02-01
影响因子：
5
通讯作者：
MODHA, P
中科院分区：
文献类型：
--
作者：
HATFIELD, RH;MENDELOW, AD;MODHA, P
Triphenyltetrazolium chloride (TTC) was used to delineate ischaemic lesions in the rat brain at various times following middle cerebral artery occlusion. A comparison was made of TTC staining by immersion and perfusion techniques and conventional light microscopy. The lesions were quantified by measuring the ischaemic area at the sections corresponding to 7 mm in front of the AO line (atlas of Konig and Klippel). In animals examined 24 h after middle cerebral artery occlusion (MCAO), the area of infarction was 17.4 +/- 1.3 mm2 on the TTC perfused slices and 17.6 +/- 1.6 mm2 on the TTC immersed slices (mean +/- SEM). By contrast, there was a marked difference between the two TTC methods when tissues were examined at shorter intervals after artery occlusion. In the TTC-perfused animals, there was no significant difference between the mean areas of infarction measured at 5-20 min, 3-4 h, or 24 h post occlusion. Immersion in TTC, however, failed to reveal any consistent ischaemic damage when applied at the earlier post-occlusion times. Conventional histopathology demonstrated minimal lesions at 5-20 min but at 4 h or more the lesions were not significantly different from those demonstrated by TTC perfusion. TTC immersion staining can, thus, only be used as a reliable marker of cerebral ischaemia damage with post-occlusion survival periods of 24 h. TTC perfusion staining gives results not significantly different from histopathology at 4 h or more post-occlusion but at earlier intervals than 24 h it differs significantly from TTC immersion staining. Thus at times of less than 24 h it may reflect mainly changes in stain perfusion, rather than ischaemic damage.
