Human organic anion transporter 4 is a renal apical organic anion/dicarboxylate exchanger in the proximal tubules
Human organic anion transporter 4 is a renal apical organic anion/dicarboxylate exchanger in the proximal tubules
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DOI：
10.1254/jphs.94.297
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发表时间：
2004-03-01
影响因子：
3.5
通讯作者：
Endou, H
中科院分区：
文献类型：
--
作者：
Ekaratanawong, S;Anzai, N;Endou, H
人有机阴离子转运蛋白OAT4在肾脏和胎盘中表达，并介导雌酮 - 3 - 硫酸盐（EIS）的高亲和力转运。由于先前的一项研究表明EIS没有反式刺激作用，通过OAT4进行有机阴离子转运的模式仍不清楚。在本研究中，我们利用稳定表达OAT4的小鼠近端肾小管细胞（S - 2 OAT4）检测了OAT4的驱动力。戊二酸（GA）抑制OAT4介导的E1S摄取（半数抑制浓度：1.25 mM），并且未标记的GA（5 mM）显著反式刺激通过S - 2 OAT4的[C - 14]GA摄取（P<......此处原文似乎不完整）
Human organic anion transporter OAT4 is expressed in the kidney and placenta and mediates high-affinity transport of estrone-3-sulfate (EIS). Because a previous study demonstrated no trans-stimulatory effects by EIS, the mode of organic anion transport via OAT4 remains still unclear. In the present study, we examined the driving force of OAT4 using mouse proximal tubular cells stably expressing OAT4 (S-2 OAT4). OAT4-mediated E1S uptake was inhibited by glutarate (GA) (IC50: 1.25 mM) and [C-14]GA uptake via S-2 OAT4 was significantly trans-stimulated by unlabeled GA (5 mM) (P