Comparative study of apoptosis-detecting techniques: TUNEL, apostain, and lamin B.
Comparative study of apoptosis-detecting techniques: TUNEL, apostain, and lamin B.
复制标题
细胞凋亡检测技术的比较研究：TUNEL、apostain 和核纤层蛋白 B。
作者：
J. Procházková;D. Kylarová;P. Vranka;V. Lichnovský
凋亡过程的机制通常被认为是高度复杂的。不幸的是，它的检测也是如此。检测灵敏度的概念不仅反映了特定技术标记大多数显示适当标志物的细胞的能力，而且反映了在凋亡过程的最早阶段标记细胞的可能性。在这项研究中，我们选择了三种技术，通过免疫组织化学可视化：末端dUTP转移酶介导的缺口末端标记（TUNEL），apostain，和核纤层蛋白B。对于大多数情况，与所使用的技术相关的观察标本中检测到的凋亡细胞数量不同。核纤层蛋白B技术的凋亡指数通常最低，TUNEL技术次之，脱辅基染色技术的凋亡指数最高。比较发现，TUNEL和apostain技术呈正相关，在大多数标本观察。TUNEL/apostain与核纤层蛋白B的线性相关性较弱。TUNEL、脱凋亡蛋白和核纤层蛋白B技术，原则上是不同的，给出了不同但相关的结果，这与它们描述在凋亡过程的不同时间段检测到的相同现象的假设是一致的。
The mechanism of the apoptotic process is generally recognized as being highly intricate. Unfortunately, so is its detection. The concept of detection sensitivity reflects not only the ability of a particular technique to label most of the cells displaying the appropriate marker but also the possibility of labeling cells in the earliest phase of the apoptotic process. For this study, we chose three techniques, visualized by means of immunohistochemistry: terminal dUTP-transferase-mediated nick end labeling (TUNEL), apostain, and lamin B. The number of apoptotic cells detected in the observed specimens with respect to the used technique differed for the majority of cases. The lowest apoptotic indices were usually obtained by the lamin B technique, the TUNEL technique followed, and the apostain technique gave the highest values. The comparison revealed that the TUNEL and apostain techniques were positively correlated in the majority of specimens observed. The linear correlation between TUNEL/apostain and lamin B was weaker. TUNEL, apostain, and lamin B techniques, variant in principle, give different yet correlating results, which is in accordance with the assumption that they describe the same phenomenon detected at different time periods of the apoptotic process.
DOI：
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发表时间：
1997-03
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
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作者：
O. Frankfurt;J. Robb;E. Sugarbaker;L. Villa
通讯作者：
O. Frankfurt;J. Robb;E. Sugarbaker;L. Villa