DNA copy number gains in head and neck squamous cell carcinoma
DNA copy number gains in head and neck squamous cell carcinoma
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DOI：
10.1038/sj.onc.1209166
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发表时间：
2006-03-02
期刊：
影响因子：
8
通讯作者：
Plass, C
中科院分区：
文献类型：
--
作者：
Lin, M;Smith, LT;Plass, C
基因扩增是癌症中癌基因激活的常见机制，已被用作鉴定新癌基因的标签。头颈部鳞状细胞癌（HNSCC）中经常观察到DNA扩增，并且已经报道了潜在的癌基因。我们应用限制性界标基因组扫描（RLGS）研究基因扩增和低水平拷贝数的变化，在HNSCC，以定位以前未表征的区域与原发性肿瘤样本中的拷贝数增益。总共对63个增强的RLGS片段进行评分，这些片段指示DNA拷贝数变化，包括单个等位基因的增加。从33个不同的染色体区域中鉴定出增强序列，包括先前报道的区域（例如3q26.3和11q13.3）以及新区域（例如3q 29、8q13.1、8q22.3、9 q32、10q24.32、14q32.32、17q25.1和20q13.33）。此外，我们的数据表明，扩增子11q13.3和3q26.3-q29可以分为可能的两个和三个独立的扩增子，分别由发表的微阵列表达数据支持的观察。
Gene amplification, a common mechanism for oncogene activation in cancer, has been used as a tag for the identification of novel oncogenes. DNA amplification is frequently observed in head and neck squamous cell carcinoma (HNSCC) and potential oncogenes have already been reported. We applied restriction landmark genome scanning (RLGS) to study gene amplifications and low-level copy number changes in HNSCC in order to locate previously uncharacterized regions with copy number gains in primary tumor samples. A total of 63 enhanced RLGS fragments, indicative of DNA copy number changes, including gains of single alleles, were scored. Enhanced sequences were identified from 33 different chromosomal regions including those previously reported (e.g. 3q26.3 and 11q13.3) as well as novel regions (e.g. 3q29, 8q13.1, 8q22.3, 9q32, 10q24.32, 14q32.32, 17q25.1 and 20q13.33). Furthermore, our data suggest that amplicons 11q13.3 and 3q26.3-q29 may be divided into possibly two and three independent amplicons, respectively, an observation supported by published microarray expression data.