Sequencing of the black rockfish chromosomal genome provides insight into spermstorage in the female ovary
Sequencing of the black rockfish chromosomal genome provides insight into spermstorage in the female ovary
复制标题
黑岩鱼染色体基因组的测序提供了对雌性卵巢中精子储存的深入了解
DOI：
10.1093/dnares/dsz023
复制
发表时间：
2019-12-01
期刊：
影响因子：
4.1
通讯作者：
Li, Jun
中科院分区：
文献类型：
--
作者：
Liu, Qinghua;Wang, Xueying;Li, Jun
黑石鱼(Sebases Schlegelii)是日本、韩国和中国等国重要的经济胎生性海洋硬骨鱼。它的特点是体内受精，精子长期储存在女性卵巢，以及高流产率。为了更好地了解受精和妊娠的机制，建立胎生硬骨鱼的参考基因组是必不可少的。在这里，我们使用太平洋生物科学Sequel，Illumina测序平台，10x基因组学，和Hi-C技术的组合，获得了包括24条染色体的848.31Mb的基因组组装，以及2.96Mb的重叠群和35.63Mb的支架N50长度。我们预测了39.98%的重复元件和26979个蛋白质编码基因。与3210万年前至5680万年前类似。此外，精子在卵巢内的存活时间长达6个月。在交配后，葡萄糖转运蛋白SLC2显示出显著的基因组选择，与碳水化合物代谢相关的KEGG通路在卵巢中显著上调。在体外，用碘乙酸钠抑制糖酵解可显著降低精子寿命。这些结果表明碳水化合物在维持精子存活率方面的重要性。破译施氏血吸虫基因组不仅为精子储存提供了新的见解，而且对海洋研究人员和生殖生物学家具有很高的价值。
Black rockfish (Sebastes schlegelii) is an economically important viviparous marine teleost in Japan, Korea, and China. It is characterized by internal fertilization, long-term sperm storage in the female ovary, and a high abortion rate. For better understanding the mechanism of fertilization and gestation, it is essential to establish a reference genome for viviparous teleosts. Herein, we used a combination of Pacific Biosciences sequel, Illumina sequencing platforms, 10x Genomics, and Hi-C technology to obtain a genome assembly size of 848.31Mb comprising 24 chromosomes, and contig and scaffold N50 lengths of 2.96 and 35.63Mb, respectively. We predicted 39.98% repetitive elements, and 26,979 protein-coding genes. S. schlegelii diverged from Gasterosteus aculeatus similar to 32.1-56.8 million years ago. Furthermore, sperm remained viable within the ovary for up to 6months. The glucose transporter SLC2 showed significantly positive genomic selection, and carbohydrate metabolism-related KEGG pathways were significantly up-regulated in ovaries after copulation. In vitro suppression of glycolysis with sodium iodoacetate reduced sperm longevity significantly. The results indicated the importance of carbohydrates in maintaining sperm survivability. Decoding the S. schlegelii genome not only provides new insights into sperm storage; additionally, it is highly valuable for marine researchers and reproduction biologists.