Folic acid conjugated magnetic iron oxide nanoparticles for nondestructive separation and detection of ovarian cancer cells from whole blood
Folic acid conjugated magnetic iron oxide nanoparticles for nondestructive separation and detection of ovarian cancer cells from whole blood
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DOI：
10.1039/c5bm00207a
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发表时间：
2016-01-01
影响因子：
6.6
通讯作者：
Xu, Hengyi
中科院分区：
文献类型：
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作者：
Liu, Wenting;Nie, Liju;Xu, Hengyi
由于缺乏早期筛查策略，卵巢癌是妇科恶性肿瘤最致命的原因。本文描述了一种使用叶酸（FA）缀合磁性氧化铁纳米颗粒（IO-FA纳米颗粒）从女性全血中分离和检测卵巢癌细胞的有效方法。通过热分解合成IO纳米颗粒，然后与FA共价结合。 IO-FA纳米颗粒通过与过度表达的叶酸受体（FR）偶联而稳定地附着在卵巢癌细胞表面，从而使细胞具有磁性。这些“磁性细胞”在磁场下从复杂的血液基质中分离出来，而没有被破坏。当尖峰卵巢癌SKOV3细胞丰度低至5 x 10 (5)%时，分离效率高达61.3%。我们还成功检测了十 (10) 名转移性卵巢癌患者的全血中的五 (5) 名。这项研究表明早期检测转移性卵巢癌细胞的可行性，这可能会在临床应用中提高卵巢癌患者的总体生存率。
Because of the lack of early screening strategies, ovarian cancer is the most deadly cause of gynecologic malignancies. This paper describes an effective method for the separation and detection of ovarian cancer cells from female whole blood, using folic acid (FA) conjugated magnetic iron oxide nanoparticles (IO-FA nanoparticles). The IO nanoparticles were synthesized by thermal decomposition and then covalently conjugated with FA. The IO-FA nanoparticles were stably attached to the surface of ovarian cancer cells by coupling to the over-expressed folate receptor (FR), thereby making the cells magnetic. These "magnetic cells" were separated from the complex blood matrix without destruction under a magnetic field. The separation efficiency was as high as 61.3% when the abundance of spiked ovarian cancer SKOV3 cells was as low as 5 x 10 (5)%. We also successfully detected five (5) out of ten (10) metastatic ovarian cancer patients' whole blood. This study suggested the feasibility of early detecting of metastatic ovarian cancer cells, which may potentially improve the ovarian cancers patients' overall survival rate for clinical applications.