An H+-coupled multidrug efflux pump, PmpM, a member of the MATE family of transporters, from Pseudomonas aeruginosa
An H+-coupled multidrug efflux pump, PmpM, a member of the MATE family of transporters, from Pseudomonas aeruginosa
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DOI：
10.1128/jb.186.1.262-265.2004
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发表时间：
2004-01-01
影响因子：
3.2
通讯作者：
Tsuchiya, T
中科院分区：
文献类型：
--
作者：
He, GX;Kuroda, T;Tsuchiya, T
我们使用药物过敏性大肠杆菌KAM32菌株作为宿主，通过PCR方法克隆了铜绿假单胞菌的基因PA1361（我们命名为基因pmpM），该基因似乎编码属于MATE家族的多药物外排泵。拥有 pmpM 基因的大肠杆菌细胞对多种抗菌剂表现出较高的耐药性。我们观察到乙锭从具有 pmpM 基因的细胞中能量依赖性流出。我们发现 PmpM 是一种 H+ 药物逆向转运蛋白，这一发现是 MATE 家族中首次报道 H+ 偶联外排泵的病例。铜绿假单胞菌中 pmpM 基因的破坏和重新引入表明 PmpM 具有功能性，并且苯扎氯铵、氟喹诺酮类、溴化乙锭、吖啶黄和氯化四苯基磷是该微生物中 PmpM 的底物。
We cloned the gene PA1361 (we designated the gene pmpM), which seemed to encode a multidrug efflux pump belonging to the MATE family, of Pseudomonas aeruginosa by the PCR method using the drug-hypersensitive Escherichia coli KAM32 strain as a host. Cells of E. coli possessing the pmpM gene showed elevated resistance to several antimicrobial agents. We observed energy-dependent efflux of ethidium from cells possessing the pmpM gene. We found that PmpM is an H+-drug antiporter, and this finding is the first reported case of an H+-coupled efflux pump in the MATE family. Disruption and reintroduction of the pmpM gene in P. aeruginosa revealed that PmpM is functional and that benzalkonium chloride, fluoroquinolones, ethidium bromide, acriflavine, and tetraphenylphosphonium chloride are substrates for PmpM in this microorganism.