Evaluation of serological cross-reactivity and cross-neutralization between the United States porcine epidemic diarrhea virus prototype and S-INDEL-variant strains.
Evaluation of serological cross-reactivity and cross-neutralization between the United States porcine epidemic diarrhea virus prototype and S-INDEL-variant strains.
复制标题
评估美国猪流行病腹泻病毒原型和S-Indel-Variant菌株之间血清学交叉反应性和交叉中和化的评估。
DOI：
10.1186/s12917-016-0697-5
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发表时间：
2016-04-05
影响因子：
2.6
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Chen Q;Thomas JT;Giménez-Lirola LG;Hardham JM;Gao Q;Gerber PF;Opriessnig T;Zheng Y;Li G;Gauger PC;Madson DM;Magstadt DR;Zhang J
在美国已经鉴定了至少两种遗传上不同的猪流行性腹泻病毒（PEDV）毒株（美国PEDV原型和S-INDEL-变体毒株）。目前兽医诊断实验室提供的用于检测PEDV特异性抗体的血清学测定是基于美国PEDV原型菌株。本研究的目的是：1）在细胞培养物中分离美国PEDV S-INDEL-变体菌株; 2）通过实验感染断奶猪产生针对美国PEDV原型和S-INDEL-变体菌株的抗血清; 3）确定各种PEDV血清学测定是否可以检测针对美国PEDV S-INDEL-变体菌株的抗体，反之亦然。在本研究中，在细胞培养物中分离美国PEDV S-INDEL-变体菌株。将三组PEDV阴性的3周龄猪（每组五头猪）经口接种美国PEDV原型分离株（先前在我们实验室中分离）、S-INDEL变体分离株或病毒阴性培养基。在接种后0、7、14、21和28天收集的血清样品通过以下PEDV血清学测定进行评价：1）使用原型和S-INDEL-变体病毒作为指示病毒的间接荧光抗体（IFA）测定; 2）针对原型和S-INDEL-变体病毒的病毒中和（VN）测试; 3）基于PEDV原型病毒株全病毒的ELISA; 4）基于PEDV原型株S1的ELISA;和5）基于PEDV S-INDEL-变体株S1的ELISA。通过IFA抗体测定和VN试验检查，针对原型菌株的阳性抗血清与原型和S-INDEL变体病毒反应并中和，并且针对S-INDEL变体菌株的阳性抗血清也与原型和S-INDEL变体病毒反应并中和。针对两种PEDV毒株的抗体可以通过所有三种ELISA检测，尽管检测率在一定程度上不同。这些数据表明，针对美国PEDV原型和S-INDEL变体菌株的抗体在体外交叉反应并交叉中和两种菌株。目前基于美国PEDV原型株的血清学测定可以检测针对两种美国PEDV株的抗体。
At least two genetically different porcine epidemic diarrhea virus (PEDV) strains have been identified in the United States (U.S. PEDV prototype and S-INDEL-variant strains). The current serological assays offered at veterinary diagnostic laboratories for detection of PEDV-specific antibody are based on the U.S. PEDV prototype strain. The objectives of this study were: 1) isolate the U.S. PEDV S-INDEL-variant strain in cell culture; 2) generate antisera against the U.S. PEDV prototype and S-INDEL-variant strains by experimentally infecting weaned pigs; 3) determine if the various PEDV serological assays could detect antibodies against the U.S. PEDV S-INDEL-variant strain and vice versa. A U.S. PEDV S-INDEL-variant strain was isolated in cell culture in this study. Three groups of PEDV-negative, 3-week-old pigs (five pigs per group) were inoculated orally with a U.S. PEDV prototype isolate (previously isolated in our lab), an S-INDEL-variant isolate or virus-negative culture medium. Serum samples collected at 0, 7, 14, 21 and 28 days post inoculation were evaluated by the following PEDV serological assays: 1) indirect fluorescent antibody (IFA) assays using the prototype and S-INDEL-variant strains as indicator viruses; 2) virus neutralization (VN) tests against the prototype and S-INDEL-variant viruses; 3) PEDV prototype strain whole virus based ELISA; 4) PEDV prototype strain S1-based ELISA; and 5) PEDV S-INDEL-variant strain S1-based ELISA. The positive antisera against the prototype strain reacted to and neutralized both prototype and S-INDEL-variant viruses, and the positive antisera against the S-INDEL-variant strain also reacted to and neutralized both prototype and S-INDEL-variant viruses, as examined by IFA antibody assays and VN tests. Antibodies against the two PEDV strains could be detected by all three ELISAs although detection rates varied to some degree. These data indicate that the antibodies against U.S. PEDV prototype and S-INDEL-variant strains cross-reacted and cross-neutralized both strains in vitro. The current serological assays based on U.S. PEDV prototype strain can detect antibodies against both U.S. PEDV strains.