Biocompatible Direct Deposition of Functionalized Nanoparticles Using Shrinking Surface Plasmonic Bubble
Biocompatible Direct Deposition of Functionalized Nanoparticles Using Shrinking Surface Plasmonic Bubble
复制标题
DOI：
10.1002/admi.202000597
复制
发表时间：
2020-06-01
影响因子：
5.4
通讯作者：
Luo, Tengfei
中科院分区：
文献类型：
--
作者：
Moon, Seunghyun;Zhang, Qiushi;Luo, Tengfei
功能化纳米粒子（NPs）是多种应用的基础。特别是在许多生物传感应用中，在不损害其生物功能的情况下将悬浮的纳米粒子聚集到表面通常是提高检测限的一个必不可少的步骤，这仍然是一个巨大的挑战。在这项工作中，利用收缩气泡展示了功能化纳米粒子在光学透明表面的生物相容性沉积。利用气泡的收缩阶段缓解了传统光热沉积技术中遇到的生物分子降解问题。沉积的纳米粒子紧密排列，并且功能分子能够在这个过程中存活下来，这通过它们强烈的荧光信号得到了验证。通过高速摄像发现，收缩气泡的收缩接触线将被接触线捕获的纳米粒子推到一个高度浓缩的区域。这种表面气泡收缩沉积（SSBD）本质上是低温的，因为在这个过程中没有添加热量。以发夹DNA功能化的金纳米粒子悬浮液作为模型系统，与光压沉积和传统的热气泡接触线沉积相比，SSBD显示出能产生更强的荧光信号。所展示的能够直接沉积功能化纳米粒子的SSBD技术可能会显著简化生物传感器的制造，从而有益于广泛的相关应用。
Functionalized nanoparticles (NPs) are the foundation of diverse applications. Especially, in many biosensing applications, concentrating suspended NPs onto a surface without deteriorating their biofunction is usually an inevitable step to improve detection limit, which remains to be a great challenge. In this work, biocompatible deposition of functionalized NPs to optically transparent surfaces is demonstrated using shrinking bubbles. Leveraging the shrinking phase of bubble mitigates the biomolecule degradation problems encountered in traditional photothermal deposition techniques. The deposited NPs are closely packed, and the functional molecules are able to survive the process as verified by their strong fluorescence signals. Using high-speed videography, it is revealed that the contracting contact line of the shrinking bubble forces the NPs captured by the contact line to a highly concentrated island. Such shrinking surface bubble deposition (SSBD) is low temperature in nature as no heat is added during the process. Using a hairpin DNA-functionalized gold NP suspension as a model system, SSBD is shown to enable much stronger fluorescence signal compared to the optical-pressure deposition and the conventional thermal bubble contact line deposition. The demonstrated SSBD technique capable of directly depositing functionalized NPs may significantly simplify biosensor fabrication and thus benefit a wide range of relevant applications.