Development of a screening method to determine the pattern of fermentation metabolites in faecal samples using on-line purge-and-trap gas chromatographic-mass spectrometric analysis
Development of a screening method to determine the pattern of fermentation metabolites in faecal samples using on-line purge-and-trap gas chromatographic-mass spectrometric analysis
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DOI：
10.1016/j.chroma.2008.12.095
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发表时间：
2009-02-27
影响因子：
4.1
通讯作者：
Verbeke, Kristin
中科院分区：
文献类型：
--
作者：
De Preter, Vicky;Van Staeyen, Greet;Verbeke, Kristin
开发了一种分析粪便样本中挥发性有机化合物（VOC）的在线筛查方法。使用吹扫捕集系统从标准溶液或粪便样品中分离 VOC，并通过 GC-MS 进行鉴定和定量。优化了实验条件并评估了系统的性能。获得线性校准曲线，相关系数至少为 0.992。日内和日间 RSD 均小于 10%。该方法成功应用于粪便样本分析，在 11 个粪便样本中鉴定出 135 种不同的挥发性有机化合物。其中，22 种 VOC 在所有志愿者中被发现，而 34 种 VOC 是针对个人的。 (C) 2009 Elsevier B.V. 保留所有权利。
An on-line screening method to analyse volatile organic compounds (VOCs) in faecal samples was developed. VOCs were isolated from a standard solution or faecal samples using a purge-and-trap system and identified and quantified by GC-MS. The experimental conditions were optimised and the performance of the system was evaluated. Linear calibration curves were obtained with correlation coefficients of at least 0.992. RSDs within and between days were less than 10%. The method was successfully applied to the analysis of faecal samples, yielding 135 different volatile organic compounds identified in 11 faecal samples. Of those, 22 VOCs were found in all volunteers, whereas 34 VOCs were person-specific. (C) 2009 Elsevier B.V. All rights reserved.