Protein quantification in the presence of poly(ethylene glycol) and dextran using the Bradford method
Protein quantification in the presence of poly(ethylene glycol) and dextran using the Bradford method
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DOI：
10.1016/j.ab.2009.07.045
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发表时间：
2009-12-01
影响因子：
2.9
通讯作者：
Marcos, Joao C.
中科院分区：
文献类型：
--
作者：
Barbosa, Helder;Slater, Nigel K. H.;Marcos, Joao C.
一些实验方法需要在聚乙二醇 (PEG) 和葡聚糖 (DEX) 等聚合物存在的情况下对蛋白质进行定量。在生物分子的水性两相系统 (ATPS) 萃取中，这些相形成聚合物对 Bradford 定量测定的干扰是众所周知的。然而，迄今为止尚未报道这些聚合物如何干扰。在本研究中，我们表明，虽然使用浓度为 20% (w/w) 的葡聚糖不会出现错误，但 PEG 浓度 > 10% (w/w) 的溶液会出现准确性损失。高于该值时，观察到测定灵敏度显着降低。 (C) 2009 Elsevier Inc. 保留所有权利。
Some experimental methodologies require the quantification of protein in the presence of polymers like poly(ethylene glycol) (PEG) and dextran (DEX). In the aqueous two-phase system (ATPS) extraction of biomolecules, the interference of these phase-forming polymers on the Bradford quantification assay is commonly recognized. However, how these polymers interfere has not been reported hitherto. In this study we show that while dextran concentrations of 20% (w/w) can be used without error, loss of accuracy occurs for solutions with PEG concentrations > 10% (w/w). Above this value a substantial decrease on the assay sensitivity is observed. (C) 2009 Elsevier Inc. All rights reserved.