Variation of carbon content among bacterial species under starvation condition
Variation of carbon content among bacterial species under starvation condition
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DOI：
10.3354/ame013113
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发表时间：
1997-07-24
影响因子：
1.4
通讯作者：
Dupuy, C
中科院分区：
文献类型：
--
作者：
Troussellier, M;Bouvy, M;Dupuy, C
为了探讨细菌生物量转换因子在水环境中的变异性，将5株海洋细菌和5株非海洋细菌分别暴露于人工海水和生理盐水(氯化钠，9g L(-1))中。根据饥饿后0、7、15和28d的细胞总数、颗粒有机碳和细胞体积(V)的测量，估算每细胞的碳含量(C-c)和单位体积碳(C)。而C-c在饥饿期间相当恒定(26.02+/-1.08 fg C cell(-1)；平均+/-标准差，n=50)，而C-v则显著增加。一般来说，海洋菌株的C-v增加比非海洋菌株更快。C-v与V无相关性，但C-v与V呈极显著正相关。这种非线性关系符合幂函数模型(r(2)=0.847，P<；0.0001)。经海洋和非海洋菌株调整后的幂函数参数没有显著差异。我们得出结论，C-v和C-c在较小程度上依赖于物种和生理状态。然而，在本研究中和其他人观察到的海洋和非海洋细菌之间的C-v差异似乎不是C-v与体积之间不同关系的结果，而更有可能是海洋细菌的低细胞体积以及它们比非海洋细菌更快地减少其水分的能力所致。
To explore the variability of bacterial biomass conversion factors in aquatic environments, 5 marine and 5 non-marine bacterial strains were exposed to nutrient deprivation in artificial seawater and physiological (NaCl, 9 g l(-1)) water, respectively. Carbon content per cell (C-c) and per volume (C) were estimated from total cell number, particulate organic carbon and cell volume (V) measurements after 0, 7, 15 and 28 d of starvation. While C-c appeared to be rather constant (26.02 +/- 1.08 fg C cell(-1); mean +/- standard error, n = 50) during starvation, C-v showed a significant increase. In general, C-v increased more rapidly in marine strains than in nonmarine strains. C-v and V were not correlated, but a highly significant relationship between C-v and V was found. The non-linear relation was well fitted by a power function model (r(2) = 0.847, P < 0.0001). Parameters of the power function adjusted to marine or non-marine strains were not significantly different. We conclude that C-v and, to a lesser extent, C-c were dependent on species and physiological status. However, it seems that the differences in C-v between marine and non-marine bacteria observed in this study and by others are not the consequence of different relationships between C-v and volume, but, more Likely, of the low cell volume of marine bacteria and their ability to reduce their water content faster than can non-marine bacteria.