Comparison of toad venoms from different Bufo species by HPLC and LC-DAD-MS/MS
Comparison of toad venoms from different Bufo species by HPLC and LC-DAD-MS/MS
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通过 HPLC 和 LC-DAD-MS/MS 比较不同蟾蜍种类的蟾蜍毒液
DOI：
10.1016/j.jep.2010.07.017
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发表时间：
2010-09-15
影响因子：
5.4
通讯作者：
Kopp, Brigitte
中科院分区：
文献类型：
--
作者：
Gao, Huimin;Zehl, Martin;Kopp, Brigitte
民族药理学相关性。蟾酥在中国被称为蟾酥，长期以来在临床上被广泛用于治疗心力衰竭、疮疡、疼痛和各种癌症。本研究的目的是探讨不同地理位置的蟾蜍毒液和相关蟾蜍种的化学差异。采用HPLC和LC-DAD-MS/MS分析了10批来自中国不同地区的商业蟾蜍毒液、1批来自Bujo bulb gargarizans的新鲜蟾蜍毒液和6批相关蟾蜍物种。根据化学成分谱及华蟾毒配基和脂蟾毒配基的含量，讨论了不同样品间化学成分的差异。结果表明，从不同样品的蟾酥甲醇提取物中共鉴定出43种化合物，除辛二酰精氨酸外，还发现了几种游离蟾蜍二烯内酯、蟾蜍二烯内酯硫酸盐和蟾蜍二烯内酯辛二酰酯。不同产地的商品蟾酥样品中，其含量在0.7%~ 10.9%之间，差异较大。在黑眶蟾蜍和海蟾蜍的样品中观察到低水平的脂蟾毒配基，但没有华蟾毒配基。甚至在绿蟾蜍样品中均未发现这两种化合物。不同商业和收集的蟾蜍毒液的化学特征显着不同，不仅在绝对和相对含量，而且在成分的数量和类型。这种变化的主要原因是物种特异性差异，但其他因素，如收获和收获后加工，以及适应不同地理位置的环境因素，似乎也有贡献。(C)2010爱思唯尔爱尔兰有限公司版权所有
Ethnopharmacological relevance. Toad venom, called Chansu in China, has been widely used for the treatment of heart failure, sores, pains, and various cancers for a long time in clinic.Aim of the study. The aim of the study is to investigate the chemical differences among a variety of toad venoms from different geographic locations and related Bufo speciesMaterials and methods. Ten batches of commercial toad venom collected from different regions in China, one batch of fresh toad venom obtained from Bujo bulb gargarizans, and six batches of related Bufo species were analyzed by HPLC and LC-DAD-MS/MS. Individual components were identified by comparison of retention times, UV spectra, and mass spectra with authentic compounds, standard addition, as well as summarized MS fragmentation rules. Based on the profile of identified constituents and the content of cinobufagin and resibufogenin, the chemical differences observed among different samples are discussedResults. Overall, 43 compounds were identified in the methanolic extracts of the different samples of toad venom Besides of suberoyl arginine, several free bufadienolides, bufadienolide sulfates, and suberoyl esters of bufadienolides were found. The total amounts of cmobufagin and resibufogenin, which are the only two control markers according to the current Chinese Pharmacopoeia, varied widely from 0 7% to 10.9% in the commercial Chansu samples collected in the different locations in China. Low levels of resibufogenin, but no cinobufagin was observed in the samples from Bufo melanosticus and Bufo marinus. and even neither of both compounds was found in the sample from Bufo viridisConclusions. The chemical profiles of the different commercial and collected toad venoms from related Bufo species differed significantly, not only in the absolute and relative contents, but also in the number and type of the constituents. The main reason for this variation are species-specific differences, but additional factors, such as the harvest and post-harvest processing, and adaption to environmental factors in different geographic locations, also seem to contribute. (C) 2010 Elsevier Ireland Ltd All rights reserved