Administration of IL-7 to normal mice stimulates B-lymphopoiesis and peripheral lymphadenopathy.
Administration of IL-7 to normal mice stimulates B-lymphopoiesis and peripheral lymphadenopathy.
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对正常小鼠施用 IL-7 会刺激 B 淋巴细胞生成和外周淋巴结肿大。
DOI：
10.4049/jimmunol.147.2.561
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发表时间：
1991
影响因子：
4.4
通讯作者：
D. Mochizuki
中科院分区：
文献类型：
--
作者：
P. Morrissey;P. Conlon;K. Charrier;S. Braddy;A. Alpert;D. Williams;A. Namen;D. Mochizuki
给正常小鼠注射IL-7 （500 ng，每天两次），持续6天，观察胸腺、脾脏、淋巴结和骨髓的细胞结构和表型组成。治疗6天后，观察到脾脏、淋巴结和骨髓细胞量明显增加，并在停药后6天内恢复到正常范围。IL-7治疗3天后，观察到B220+/表面IgM-骨髓细胞数量增加。治疗6天后，这些数字进一步增加，脾脏中观察到大量B220+/sIgM-细胞。il -7处理小鼠的c mu+/sIgM-细胞数量也增加。分析B220和BP-1在sIgM-骨髓细胞上的表达，IL-7处理后B220+/BP-1+群体显著增加，且B220+/BP-1-群体的大小与对照小鼠无显著差异。停止IL-7治疗后，前b细胞数量迅速下降。IL-7治疗6天后，脾脏和淋巴结的B细胞数量增加了两倍。停用IL-7后6天内B细胞数量降至正常值。在il -7处理小鼠的脾脏中，具有不成熟表型（如sIgMhi/sIgDlo， Ia和FcR表达水平降低）的B细胞数量显著增加。il -7处理小鼠的淋巴结和脾脏中CD8+和CD4+ T细胞的数量也增加。在停止IL-7治疗后，这些数字下降到正常水平。
Normal mice were injected with IL-7 (500 ng, twice daily) for various periods of time up to 6 days and the cellularity and phenotypic composition of the thymus, spleen, lymph node, and bone marrow was assessed. After 6 days of treatment, significant increases in the cellularity of the spleen, lymph node, and bone marrow were observed which returned to the normal range within 6 days after cessation of treatment. After 3 days of IL-7 treatment, increased numbers of B220+/surface(s) IgM- bone marrow cells were observed. After 6 days of treatment, these numbers were still further increased and a significant population of B220+/sIgM- cells were observed in the spleen. The numbers of c mu+/sIgM- cells were also increased in the IL-7-treated mice. Analysis of the expression of B220 and BP-1 on the sIgM- bone marrow cells revealed that the B220+/BP-1+ population was dramatically increased after IL-7 treatment and the size of the B220+/BP-1- population did not differ from control mice. The pre-B cell numbers declined rapidly after the cessation of IL-7 treatment. After 6 days of IL-7 treatment, a twofold increase in the number of B cells in the spleen and lymph node was observed. The B cell numbers declined to normal values within 6 days after the cessation of IL-7 administration. In the spleens of the IL-7-treated mice, there was a significant increase in the number of B cells with an immature phenotype (e.g., sIgMhi/sIgDlo, decreased levels of Ia and FcR expression). The numbers of CD8+ and CD4+ T cells were also increased in the lymph node and spleen of the IL-7-treated mice. These numbers declined to normal levels after the cessation of IL-7 treatment.