Plasma angiogenesis and oxidative stress markers in patients with diabetic retinopathy
Plasma angiogenesis and oxidative stress markers in patients with diabetic retinopathy
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DOI：
10.1080/1354750x.2020.1774654
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发表时间：
2020-07-03
期刊：
影响因子：
2.6
通讯作者：
Varma, Muralidhar
中科院分区：
文献类型：
--
作者：
Gaonkar, Bhaskar;Prabhu, Krishnananda;Varma, Muralidhar
背景：糖尿病视网膜病变（DR）的发病机制与视网膜新生血管形成和高血压诱导的氧化应激有关。在这项研究中，我们假设与这些紊乱相关的血浆血管生成和氧化应激标志物可以帮助筛查糖尿病患者，这些患者发生视网膜病变的风险增加。方法：本研究包括正常人（n = 148）、2型糖尿病无视网膜病变（DNR;n = 148）、增殖性DR（PDR;n = 74）和非PDR（NPDR;n = 148）受试者。检测血浆血管内皮生长因子-A（VEGF-A）、缺氧诱导因子-1 α（HIF-1 α）、基质金属蛋白酶-9（MMP-9）、色素上皮衍生因子（PEDF）、一氧化氮（NO）、可溶性糖基化终产物受体（sICAM-1）、丙二醛（MDA）和蛋白巯基。结果：在两个视网膜病变组中，促血管生成因子和氧化应激标志物的血浆浓度均观察到统计学显著性增加。相反，这些组中抗血管生成因子和抗氧化剂的浓度显著降低。受试者工作特征分析表明，HIF-1 α和PEDF的血浆阈值可以作为NPDR的合适标志物。而在PDR中，HIF-1 α、NO、MMP-9和PEDF具有较高的敏感性和特异性。结论：缺氧、基质降解、血管生成抑制等因素在预测DR中起重要作用。
Background:Neovascularization in the retina and hyperglycaemia-induced oxidative stress are implicated in the pathogenesis of diabetic retinopathy (DR). In this study, we hypothesized that the plasma angiogenic and oxidative stress markers associated with these derangements could aid in the screening of diabetic patients who are at an increased risk of developing retinopathy. Methods:This study included normal (n = 148), type2 diabetes without retinopathy (DNR;n = 148), proliferative DR (PDR;n = 74) and non-PDR (NPDR;n = 148) subjects. Plasma concentrations of vascular endothelial growth factor-A (VEGF-A), hypoxia-inducible factor-1 alpha (HIF-1 alpha), matrix metalloproteinase-9 (MMP-9), pigment epithelium-derived factor (PEDF), nitric oxide (NO), soluble receptors for advanced glycation end products (sRAGE), malondialdehyde (MDA) and protein thiols were estimated. Results:A statistically significant increase was observed in the plasma concentrations of pro-angiogenic factors and markers of oxidative stress in both retinopathy groups. By contrast, the concentrations of anti-angiogenic factors and antioxidants were decreased significantly in these groups. Receiver operating characteristic analysis indicated that the plasma thresholds of HIF-1 alpha and PEDF can be suitable markers in case of NPDR. However, in PDR, HIF-1 alpha, NO, MMP-9 and PEDF showed high sensitivity and specificity. Conclusions:The factors associated with hypoxia, matrix degradation and angiogenic inhibition play a crucial role in predicting DR.